Supplementary Materials

Supplementary Materials for:

Trans-inhibition of activation and proliferation signals by Fc receptors in mast cells and basophils

Odile Malbec, Lydie Cassard, Marcello Albanesi, Friederike Jönsson, David Mancardi, Gaëtan Chicanne, Bernard Payrastre, Patrice Dubreuil, Eric Vivier, Marc Daëron*

*Corresponding author. Email: marc.daeron{at}

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  • Fig. S1. FcεRI are engaged independently by mIgE anti-DNP 2682-I and rIgE IR162.
  • Fig. S2. Mouse and rat IgE are endocytosed independently.
  • Fig. S3. FcγRIIB-dependent cis-inhibition in BMMCs.
  • Fig. S4. FcγRIIB-dependent trans-inhibition of mast cell activation by IgE antibodies of several specificities.
  • Fig. S5. MAR IgG does not prevent the binding of DNP-BSA to mIgE anti-DNP.
  • Fig. S6. Trans-inhibition is reversible in BMMCs.
  • Fig. S7. Schematic representation of the major FcεRI-dependent signaling pathways.
  • Fig. S8. Cis-inhibition in RBL transfectants.
  • Fig. S9. MMC-1 cells express Kit, FcγRIIB, and FcγRIIIA.
  • Fig. S10. The oncogene v-Abl accounts for the autonomous proliferation of MMC-1 cells.
  • Fig. S11. Cis-inhibition in mouse and human basophils.
  • Fig. S12. Trans-inhibition in human basophils.
  • Fig. S13. Trans-inhibition persists for several days in human basophils.
  • Fig. S14. Human basophils still respond to PMA and ionomycin 48 hours after they were subjected to trans-inhibition.
  • Fig. S15. Schematic representation of trans-inhibition during co- and autoregulation of FcεRI signaling.

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Citation: O. Malbec, L. Cassard, M. Albanesi, F. Jönsson, D. Mancardi, G. Chicanne, B. Payrastre, P. Dubreuil, E. Vivier, M. Daëron, Trans-inhibition of activation and proliferation signals by Fc receptors in mast cells and basophils. Sci. Signal. 9, ra126 (2016).

© 2016 American Association for the Advancement of Science