Supplementary Materials

Supplementary Materials for:

Reduced abundance of the E3 ubiquitin ligase E6AP contributes to decreased expression of the INK4/ARF locus in non–small cell lung cancer

Cristina Gamell, Twishi Gulati, Yaara Levav-Cohen, Richard J. Young, Hongdo Do, Pat Pilling, Elena Takano, Neil Watkins, Stephen B. Fox, Prudence Russell, Doron Ginsberg, Brendon J. Monahan, Gavin Wright, Alex Dobrovic, Sue Haupt, Ben Solomon, Ygal Haupt*

*Corresponding author. Email: ygal.haupt{at}petermac.org

This PDF file includes:

  • Fig. S1. E6AP does not affect Cdc6 transcript stability.
  • Fig. S2. Immunoblot of cell lysates from Fig. 3A.
  • Fig. S3. E3 ligase catalytic activity of E6AP is not required for inhibition of E2F1 transcriptional activity.
  • Fig. S4. The effect of E6AP on E2F1 targets is specific.
  • Fig. S5. E6AP binds to the N-terminal domain of E2F1.
  • Fig. S6. E6AP and pRb inhibit E2F1 transcriptional activity in a nonsynergistic and nonadditive manner.
  • Fig. S7. Changes in E2F1 abundance did not contribute to the effect of E6AP on CDC6 expression.
  • Fig. S8. Low E6AP mRNA expression is not associated with clinical outcome in patients with stage III lung cancer.
  • Fig. S9. Expression of UBE3A in lung adenocarcinoma NSCLC patients.
  • Table S1. List of primers used.

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Citation: C. Gamell, T. Gulati, Y. Levav-Cohen, R. J. Young, H. Do, P. Pilling, E. Takano, N. Watkins, S. B. Fox, P. Russell, D. Ginsberg, B. J. Monahan, G. Wright, A. Dobrovic, S. Haupt, B. Solomon, Y. Haupt, Reduced abundance of the E3 ubiquitin ligase E6AP contributes to decreased expression of the INK4/ARF locus in non–small cell lung cancer. Sci. Signal. 10, eaaf8223 (2017).

© 2017 American Association for the Advancement of Science