Supplementary Materials
Targeting TNFR2 with antagonistic antibodies inhibits proliferation of ovarian cancer cells and tumor-associated Tregs
Heather Torrey, John Butterworth, Toshiyuki Mera, Yoshiaki Okubo, Limei Wang, Danielle Baum, Audrey Defusco, Sara Plager, Sarah Warden, Daniel Huang, Eva Vanamee, Rosemary Foster, Denise L. Faustman*
*Corresponding author. Email: faustman{at}helix.mgh.harvard.edu
This PDF file includes:
- Fig. S1. TNFR2 antagonists inhibit Treg proliferation in the presence of IL-2 and TNF.
- Fig. S2. TNFR2 antagonists inhibit Treg expansion by TNF.
- Fig. S3. Functional activity of TNFR2 antagonists is independent of Fc region or receptor cross-linking.
- Fig. S4. Inhibition of cell division limits the efficacy of TNFR2 antagonist inhibition of Tregs.
- Fig. S5. Representative FACS dot plot of OVCAR3 live/dead staining analysis and TNFR2 oncogene abundance.
- Fig. S6. Treatment of CD4+ cells with TNFR2 antagonistic antibodies reduces NF- κB activation.
Technical Details
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Citation: H. Torrey, J. Butterworth, T. Mera, Y. Okubo, L. Wang, D. Baum, A. Defusco, S. Plager, S. Warden, D. Huang, E. Vanamee, R. Foster, D. L. Faustman, Targeting TNFR2 with antagonistic antibodies inhibits proliferation of ovarian cancer cells and tumor-associated Tregs. Sci. Signal. 10, eaaf8608 (2017).