Supplementary Materials

Supplementary Materials for:

Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes

Matthew A. Nystoriak, Madeline Nieves-Cintrón, Tommaso Patriarchi, Olivia R. Buonarati, Maria Paz Prada, Stefano Morotti, Eleonora Grandi, Julia Dos Santos Fernandes, Katherine Forbush, Franz Hofmann, Kent C. Sasse, John D. Scott, Sean M. Ward, Johannes W. Hell, Manuel F. Navedo*

*Corresponding author. Email: mfnavedo{at}ucdavis.edu

This PDF file includes:

  • Fig. S1. CaV1.2 channels do not associate with TfRs or ANO1 in isolated murine arterial myocytes.
  • Fig. S2. Wild-type and mutant AKAP150.
  • Fig. S3. Glucose-induced potentiation of nifedipine-sensitive Ba2+ currents in arterial myocytes of different vascular beds.
  • Fig. S4. Enhanced L-type Ba2+ currents in 20 mM ᴅ-glucose require glucose transport through GLUT4 and metabolically active glucose enantiomer.
  • Fig. S5. I-V relationship in AKAP150−/− arterial myocytes in 10 and 20 mM ᴅ-glucose.
  • Fig. S6. Dependence of changes in global [Ca2+]i on metabolically active glucose enantiomer and CaV1.2 channels.
  • Fig. S7. Basal abundances of KV2.1, BKCa α, and BKCa β1 subunits are similar in arteries from wild-type and S1928A mice.
  • Fig. S8. Inhibition of O-GlcNAc does not affect 20 mM ᴅ-glucose stimulation of CaV1.2 channels.
  • Fig. S9. Glucose-induced vasoconstriction is similar in intact and endothelium-denuded arteries from wild-type mice.
  • Fig. S10. Representative IBa recordings.
  • Fig. S11. Enhanced vascular tone in arteries from mice on HFD requires CaV1.2 channel activity.
  • Fig. S12. A subpopulation of CaV1.2 associates with PKAcat in human arterial myocytes.
  • Table S1. Body weight and nonfasting blood glucose for LFD and HFD mice.
  • Table S2. K+-induced constriction and passive diameters of arteries from wild-type and S1928A LFD and HFD mice.
  • Table S3. Age, gender, and metabolic state of the human subjects.

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Citation: M. A. Nystoriak, M. Nieves-Cintrón, T. Patriarchi, O. R. Buonarati, M. P. Prada, S. Morotti, E. Grandi, J. D. S. Fernandes, K. Forbush, F. Hofmann, K. C. Sasse, J. D. Scott, S. M. Ward, J. W. Hell, M. F. Navedo, Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes. Sci. Signal. 10, eaaf9647 (2017).

© 2017 American Association for the Advancement of Science