Supplementary Materials

Supplementary Materials for:

AMPK promotes mitochondrial biogenesis and function by phosphorylating the epigenetic factors DNMT1, RBBP7, and HAT1

Traci L. Marin, Brendan Gongol, Fan Zhang, Marcy Martin, David A. Johnson, Han Xiao, Yinsheng Wang, Shankar Subramaniam, Shu Chien, John Y.-J. Shyy*

*Corresponding author. Email: jshyy{at}ucsd.edu

This PDF file includes:

  • Fig. S1. Predicted AMPK-regulated epigenetic network.
  • Fig. S2. Phosphorylation of RBBP7 and DNMT1 by AMPK.
  • Fig. S3. Confirmation of pharmacologically induced AMPK activation and siRNA efficacy and time course analysis of RBBP7 interaction with DNMT1 and HAT1.
  • Fig. S4. AMPK activation decreased UCP2 and UCP3 promoter methylation.
  • Fig. S5. AMPK activation decreased promoter methylation in an AMPK- and RBBP7-dependent manner.
  • Fig. S6. AMPK activation enhanced NRF1, NRF2, UCP2, and UCP3 promoter histone acetylation through HAT1 and RBBP7.
  • Fig. S7. AMPK activation decreased nucleosomal compaction of PGC-1α, NRF1, NRF2, Tfam, UCP2, and UCP3 genes.
  • Fig. S8. AMPK activation decreased nucleosomal compaction in promoters of NRF1, NRF2, UCP2, and UCP3, resulting in increased translation.
  • Fig. S9. Pulsatile shear stress decreased nucleosomal compaction of PGC-1α, NRF1, NRF2, Tfam, UCP2, and UCP3 genes.
  • Fig. S10. AICAR regulated nucleosome remodeling and increased gene expression of UCP2 and UCP3 through AMPKα2 in vivo.
  • Table S1. AMPK phosphorylation sequence containing proteins predicted to be involved in epigenetic regulation.
  • Table S2. Primers used in methylation, FAIRE, ChIP, and mRNA quantification.

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Citation: T. L. Marin, B. Gongol, F. Zhang, M. Martin, D. A. Johnson, H. Xiao, Y. Wang, S. Subramaniam, S. Chien, J. Y.-J. Shyy, AMPK promotes mitochondrial biogenesis and function by phosphorylating the epigenetic factors DNMT1, RBBP7, and HAT1. Sci. Signal. 10, eaaf7478 (2017).

© 2017 American Association for the Advancement of Science