Supplementary Materials for:

PDZRN3 destabilizes endothelial cell-cell junctions through a PKCζ-containing polarity complex to increase vascular permeability

Raj N. Sewduth, Héléna Kovacic, Béatrice Jaspard-Vinassa, Vincent Jecko, Thomas Wavasseur, Nicolas Fritsch, Mathieu Pernot, Sylvie Jeaningros, Etienne Roux, Pascale Dufourcq, Thierry Couffinhal, Cécile Duplàa*

*Corresponding author. Email: cecile.duplaa{at}inserm.fr

This PDF file includes:

• Fig S1. Ectopic expression of Pdzrn3 in embryos.
• Fig S2. Immunostaining of PDZRN3 in vessels in brain sections.
• Fig S3. Analysis of Pdzrn3 depletion in isolated endothelial cells.
• Fig. S4. Analysis of mutant and control mouse brain vasculature by micro-CT.
• Fig S5. Quantitative RT-PCR analysis of Wnt3a, Wnt5a, Wnt7a, and Wnt7b expression after MCAO.
• Fig. S6. Knockdown efficiency of siRNAs directed against Pdzrn3, Pkcζ, and MUPP1.
• Fig S7. Schematic figure.

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Citation: R. N. Sewduth, H. Kovacic, B. Jaspard-Vinassa, V. Jecko, T. Wavasseur, N. Fritsch, M. Pernot, S. Jeaningros, E. Roux, P. Dufourcq, T. Couffinhal, C. Duplàa, PDZRN3 destabilizes endothelial cell-cell junctions through a PKCζ-containing polarity complex to increase vascular permeability. Sci. Signal. 10, eaag3209 (2017).

© 2017 American Association for the Advancement of Science