Supplementary Materials

Supplementary Materials for:

A cytoplasmic role of Wnt/β-catenin transcriptional cofactors Bcl9, Bcl9l, and Pygopus in tooth enamel formation

Claudio Cantù, Pierfrancesco Pagella, Tania D. Shajiei, Dario Zimmerli, Tomas Valenta, George Hausmann, Konrad Basler,* Thimios A. Mitsiadis*

*Corresponding author. Email: konrad.basler{at}imls.uzh.ch (K.B.); thimios.mitsiadis{at}zzm.uzh.ch (T.A.M.)

This PDF file includes:

  • Fig. S1. Bcl9 and Pygo2 are present in differentiated, secretory ameloblasts but not in the stem cell niche.
  • Fig. S2. β-Catenin transcriptional output is required for early tooth development.
  • Fig. S3. K14-Cre–mediated deletion of Bcl9/9l does not cause early tooth development arrest.
  • Fig. S4. The Bcl9/9l-Pygo1/2 interaction is required for proper enamel formation.
  • Fig. S5. Ameloblast-specific Bcl9 interactors.
  • Fig. S6. Bcl9 and Bcl9l are present in the cytoplasm along the secretory pathway.
  • Fig. S7. Bcl9 and Bcl9l colocalize with the secretory pathway proteins calnexin and COP-I.
  • Table S1. Proteins found in two independent pulldown experiments using an antibody recognizing Bcl9 and subtracting the proteins retrieved by pulldown with
    control preimmune IgG.

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Citation: C. Cantù, P. Pagella, T. D. Shajiei, D. Zimmerli, T. Valenta, G. Hausmann, K. Basler, T. A. Mitsiadis, A cytoplasmic role of Wnt/β-catenin transcriptional cofactors Bcl9, Bcl9l, and Pygopus in tooth enamel formation. Sci. Signal. 10, eaah4598 (2017).

© 2017 American Association for the Advancement of Science