Supplementary Materials

Supplementary Materials for:

A high-throughput, image-based screen to identify kinases involved in brown adipocyte development

Aliki Perdikari, Elisabeth Kulenkampff, Carla Rudigier, Heike Neubauer, Gerd Luippold, Norbert Redemann, Christian Wolfrum*

*Corresponding author. Email: christian-wolfrum{at}ethz.ch

This PDF file includes:

  • Fig. S1. High-throughput and high-content image-based kinase screening to identify kinases that play a role in brown adipocyte development.
  • Fig. S2. Verification of lentiviral-mediated shRNA knockdown of genes encoding the α1, α2, β1, β2, and γ1 subunits of AMPK.
  • Fig. S3. Verification of lentiviral-mediated shRNA knockdown of genes encoding the γ2 and γ3 subunits of AMPK.
  • Fig. S4. Analysis building blocks to identify UCP1-positive cells in UCP1 immunofluorescence images.
  • Fig. S5. Analysis building blocks to assess the differentiation of brown mature adipocytes.

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.74 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). MISSION shRNA mouse gene family, SM0121.
  • Table S2 (Microsoft Excel format). Select Protein Kinase Inhibitor Library I, 539744.
  • Table S3 (Microsoft Excel format). Select Protein Kinase Inhibitor Library II, 539745.
  • Table S4 (Microsoft Excel format). Select Protein Kinase Inhibitor Library III, 539746.
  • Data file S1 (Microsoft Excel format). Statistical analysis of the kinase screening results for relative proliferation, differentiation, and formation.
  • Data file S2 (Microsoft Excel format). Statistical analysis of the kinase screening results for relative formation using PPARγ shRNA–mediated knockdown as negative control.
  • Data file S3 (Microsoft Excel format). Statistical analysis of the kinase inhibitor screening results for relative proliferation, differentiation, and formation.
  • Data file S4 (Microsoft Excel format). Lentiviral-mediated shRNA kinase screening.
  • Data file S5 (Microsoft Excel format). Kinase inhibitor screening.
  • Data file S6 (Microsoft Excel format). Control values for lentiviral shRNA screening for replicates A and B.
  • Data file S7 (Microsoft Excel format). Control values for inhibitor screening for replicates A and B.

[Download Tables S1 to S4]

[Download Data Files S1 to S7]


Citation: A. Perdikari, E. Kulenkampff, C. Rudigier, H. Neubauer, G. Luippold, N. Redemann, C. Wolfrum, A high-throughput, image-based screen to identify kinases involved in brown adipocyte development. Sci. Signal. 10, eaaf5357 (2017).

© 2017 American Association for the Advancement of Science