Supplementary Materials

Supplementary Materials for:

Identification of a selective small-molecule inhibitor of type 1 adenylyl cyclase activity with analgesic properties

Tarsis F. Brust, Doungkamol Alongkronrusmee, Monica Soto-Velasquez, Tanya A. Baldwin, Zhishi Ye, Mingji Dai, Carmen W. Dessauer, Richard M. van Rijn, Val J. Watts*

*Corresponding author. Email: wattsv{at}purdue.edu

This PDF file includes:

  • Fig. S1. Inhibitory activity of ST034307 and NB001 against AC1.
  • Fig. S2. Inhibitory activity of ST034307 after pertussis toxin treatment or sequestration of Gβγ.
  • Fig. S3. A23187- and forskolin-stimulated cAMP accumulation in HEK-AC1 cells.
  • Table S1. Inhibition of A23187-stimulated AC1 activity using two different methods of measuring cAMP accumulation.
  • Legend for data S1

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Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Data S1 (Microsoft Excel format). NDL-3000 screen results.

Citation: T. F. Brust, D. Alongkronrusmee, M. Soto-Velasquez, T. A. Baldwin, Z. Ye, M. Dai, C. W. Dessauer, R. M. van Rijn, V. J. Watts, Identification of a selective small-molecule inhibitor of type 1 adenylyl cyclase activity with analgesic properties. Sci. Signal. 10, eaah5381 (2017).

© 2017 American Association for the Advancement of Science