Supplementary Materials

Supplementary Materials for:

Transcriptional activation of lipogenesis by insulin requires phosphorylation of MED17 by CK2

Jose A. Viscarra, Yuhui Wang, Il-Hwa Hong, Hei Sook Sul*

*Corresponding author. Email: hsul{at}berkeley.edu

This PDF file includes:

  • Fig. S1. Coimmunoprecipitation experiments with USF1 and Mediator subunits.
  • Fig. S2. Additional experiments for ChIP assays in transfected 293FT cells.
  • Fig. S3. MED6 and MED7 ChIP experiments using HepG2 cells.
  • Fig. S4. Relative abundance of FASN, USF1, and MED17 mRNA and protein in mice and cells.
  • Fig. S5. Immunoblot of phosphoserine and MED17 from liver nuclear extracts.
  • Fig. S6. In vitro phosphorylation assay for MED17 using p38 MAPK.
  • Fig. S7. Triglyceride accumulation in HepG2 cells after knockdown of MED17 or CK2.
  • Fig. S8. Adenovirally mediated overexpression of MED17 and rescue experiments.
  • Fig. S9. FASN protein abundance in the livers of shMED17-, shCK2-, or S53A-adenovirus–infected mice.
  • Fig. S10. MED17 phosphorylation and FASN mRNA abundance in the liver of ob/ob mice.
  • Table S1. Primers used for ChIP.

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Citation: J. A. Viscarra, Y. Wang, I.-H. Hong, H. S. Sul, Transcriptional activation of lipogenesis by insulin requires phosphorylation of MED17 by CK2. Sci. Signal. 10, eaai8596 (2017).

© 2017 American Association for the Advancement of Science