Supplementary Materials

Supplementary Materials for:

Identifying protein kinase–specific effectors of the osmostress response in yeast

Natalie Romanov, David Maria Hollenstein, Marion Janschitz, Gustav Ammerer, Dorothea Anrather, Wolfgang Reiter*

*Corresponding author. Email: wolfgang.l.reiter{at}

This PDF file includes:

  • Fig. S1. Data from the setup SR are consistent with data described in previous studies.
  • Fig. S2. Effects caused by Kss1 cross-talk are negligible.
  • Fig. S3. Tsl1 is a target of Hog1.
  • Fig. S4. Rck2 is the effector kinase of HOG signaling.
  • Fig. S5. Network hubs of NetworKIN-predicted interactions of kinases possibly involved in the regulation of the indirect targets of Hog1.
  • Fig. S6. Nth1 might be a target of Hog1.
  • Fig. S7. M-track whole-cell extract measurements (complete data set).
  • Fig. S8. Serial dilution droplet test of deletion mutants (complete data set).
  • Legends for tables S1 to S8
  • References (111, 112)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Peptide list over each separate replicate experiment.
  • Table S2 (Microsoft Excel format). Summary of quantified phosphorylation sites over all MS experiments.
  • Table S3 (Microsoft Excel format). Number of peptides in different fields per protein.
  • Table S4 (Microsoft Excel format). Inhibitor-susceptible S/T-P motifs (not assigned in setup SR) covered in only one of the setups.
  • Table S5 (Microsoft Excel format). Putative indirect targets of Hog1.
  • Table S6 (Microsoft Excel format). Strains used in this study.
  • Table S7 (Microsoft Excel format). Plasmids used in this study.
  • Table S8 (Microsoft Excel format). Details of the individual experimental setups.

Citation: N. Romanov, D. M. Hollenstein, M. Janschitz, G. Ammerer, D. Anrather, W. Reiter, Identifying protein kinase–specific effectors of the osmostress response in yeast. Sci. Signal. 10, eaag2435 (2017).

© 2017 American Association for the Advancement of Science