Supplementary Materials

Supplementary Materials for:

Cytoskeletal adaptivity regulates T cell receptor signaling

Timothy J. Thauland, Kenneth H. Hu, Marc A. Bruce, Manish J. Butte*

*Corresponding author. Email: mbutte{at}mednet.ucla.edu

This PDF file includes:

  • Fig. S1. Ca2+ flux measurements in naïve and effector T cells.
  • Fig. S2. AFM measurements of T cell stiffness.
  • Fig. S3. Naïve and effector T cells have similar amounts of F-actin.
  • Fig. S4. Inhibiting ROCK reduces cofilin phosphorylation and softens naïve T cells.
  • Fig. S5. Inhibiting ROCK enhances Ca2+ flux in naïve T cells.
  • Fig. S6. Inhibiting LIMK reduces cofilin phosphorylation and enhances T cell activation.
  • Fig. S7. Time course of the activation-induced softening of T cells.
  • Fig. S8. Cofilin controls immune synapse size and T cell activation.
  • Fig. S9. Examples of immune synapses formed by control T cells and by ROCK CAT–expressing T cells.
  • Fig. S10. Model for the control of immune synapse size in T cells by the RhoA-ROCK-LIMK-cofilin axis.
  • Fig. S11. The cantilever tip is unaffected by repeated scanning.

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Citation: T. J. Thauland, K. H. Hu, M. A. Bruce, M. J. Butte, Cytoskeletal adaptivity regulates T cell receptor signaling. Sci. Signal. 10, eaah3737 (2017).

© 2017 American Association for the Advancement of Science