Supplementary Materials

Supplementary Materials for:

RAF1/BRAF dimerization integrates the signal from RAS to ERK and ROKα

Andrea Varga, Karin Ehrenreiter, Bertram Aschenbrenner, Pawel Kocieniewski, Marek Kochanczyk, Tomasz Lipniacki, Manuela Baccarini*

*Corresponding author. Email: manuela.baccarini{at}

This PDF file includes:

  • Text S1. Computational model description
  • Text S2. Species definition—Proteins and their sites or domains
  • Text S3. Model parameters
  • Text S4. Simulation protocols
  • Text S5. Parameter sensitivity analysis
  • Text S6. Site perturbations
  • Fig. S1. Impact of phosphoablative and phosphomimetic mutations on the interaction of full-length RAF with full-length ROKα.
  • Fig. S2. The kinetics of ERK phosphorylation in EGF-stimulated MEFs are consistent with the MEK/ERK phosphorylation profiles generated by mathematical modeling.
  • Fig. S3. Parameter sensitivity analysis and site perturbation.
  • Table S1. Model parameters.
  • Legend for Code S1
  • References (4956)

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.25 MB

Other Supplementary Material for this manuscript includes the following:

  • Code S1 (.bngl format). A text file with computer-readable model implementation written in BNGL.

Citation: A. Varga, K. Ehrenreiter, B. Aschenbrenner, P. Kocieniewski, M. Kochanczyk, T. Lipniacki, M. Baccarini, RAF1/BRAF dimerization integrates the signal from RAS to ERK and ROKα. Sci. Signal. 10, eaai8482 (2017).

© 2017 American Association for the Advancement of Science