Supplementary Materials

Supplementary Materials for:

Phosphorylation of the exocyst protein Exo84 by TBK1 promotes insulin-stimulated GLUT4 trafficking

Maeran Uhm, Merlijn Bazuine, Peng Zhao, Shian-Huey Chiang, Tingting Xiong, Sheelarani Karunanithi, Louise Chang, Alan R. Saltiel*

*Corresponding author. Email: asaltiel{at}ucsd.edu

This PDF file includes:

  • Fig. S1. TBK1 activity is required for insulin-stimulated GLUT4 trafficking.
  • Fig. S2. TBK1 phosphorylates Exo84 both in vitro and in cells.
  • Fig. S3. TBK1 directly interacts with Exo84 through the coiled-coil domain of TBK1.
  • Fig. S4. TBK1 does not affect RalA activity.
  • Fig. S5. Mass spectrometry sequence coverage of affinity-purified phosphorylated HA-Exo84.
  • Fig. S6. Temporal dynamics of the TBK1/exocyst/RalA complex in 3T3-L1 adipocytes in response to insulin.

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Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Data files S1 and S2 (Microsoft Excel format). Identified phosphorylation sites of Exo84 from mass spectrometry.

Citation: M. Uhm, M. Bazuine, P. Zhao, S.-H. Chiang, T. Xiong, S. Karunanithi, L. Chang, A. R. Saltiel, Phosphorylation of the exocyst protein Exo84 by TBK1 promotes insulin-stimulated GLUT4 trafficking. Sci. Signal. 10, eaah5085 (2017).

© 2017 American Association for the Advancement of Science