Supplementary Materials

Supplementary Materials for:

Sphingosine 1-phosphate signaling through its receptor S1P5 promotes chromosome segregation and mitotic progression

Guillaume Andrieu, Adeline Ledoux, Sophie Branka, Magalie Bocquet, Julia Gilhodes, Thierry Walzer, Kousuke Kasahara, Masaki Inagaki, Roger A. Sabbadini, Olivier Cuvillier,* Anastassia Hatzoglou*

*Corresponding author. Email: olivier.cuvillier{at}inserm.fr (O.C.); anastassia.hatzoglou{at}ipbs.fr (A.H.)

This PDF file includes:

  • Fig. S1. SphK1-S1P pathway controls mitotic progression in RPE1 cells.
  • Fig. S2. S1P dose effect on mitotic progression.
  • Fig. S3. S1P does not regulate entry into mitosis.
  • Fig. S4. SphK1 overexpression leads to chromosome missegregation.
  • Fig. S5. S1P receptor mRNA expression in different cell lines.
  • Fig. S6. Role of VPC23019, JTE013, or PT in S1P-induced ERK phosphorylation and mitotic index.
  • Fig. S7. S1P receptor abundance in WT or S1P5−/− MEFs.
  • Fig. S8. SphK2 regulates mitotic progression.
  • Table S1. List of antibodies.
  • Legends for movies S1 and S2

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.47 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Representative movie showing mitosis in a GFPexpressing cell.
  • Movie S2 (.avi format). Representative movie showing mitosis in a GFP-SphK1–expressing cell.

[Download Movies S1 and S2]


Citation: G. Andrieu, A. Ledoux, S. Branka, M. Bocquet, J. Gilhodes, T. Walzer, K. Kasahara, M. Inagaki, R. A. Sabbadini, O. Cuvillier, A. Hatzoglou, Sphingosine 1-phosphate signaling through its receptor S1P5 promotes chromosome segregation and mitotic progression.. Sci. Signal. 10, eaah4007 (2017).

© 2017 American Association for the Advancement of Science