Supplementary Materials

Supplementary Materials for:

The conformational state of hERG1 channels determines integrin association, downstream signaling, and cancer progression

Andrea Becchetti, Silvia Crescioli, Francesca Zanieri, Giulia Petroni, Raffaella Mercatelli, Stefano Coppola, Luca Gasparoli, Massimo D'Amico, Serena Pillozzi, Olivia Crociani, Matteo Stefanini, Antonella Fiore, Laura Carraresi, Virginia Morello, Sagar Manoli, Maria Felice Brizzi, Davide Ricci, Mauro Rinaldi, Alessio Masi, Thomas Schmidt, Franco Quercioli, Paola Defilippi, Annarosa Arcangeli*

*Corresponding author. Email: annarosa.arcangeli{at}unifi.it

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Characterization of HEK 293 and HCT116 cells transfected with KCNE1.
  • Fig. S2. Coimmunoprecipitation of HEK-hERG1 cells seeded on fibronectin.
  • Fig. S3. Characterization of HEK CFPhERG1+β1YFP cells.
  • Fig. S4. Fluorescence decay of a donor control sample.
  • Fig. S5. Control experiments to validate FRET results.
  • Fig. S6. Protocol for image acquisition of HEK 293 cells.
  • Fig. S7. Background correction and FRET map calculation.
  • Fig. S8. FRET efficiency as a function of the ratio of donor to acceptor.
  • Fig. S9. Lymph node metastases in mice orthotopically injected with MDA-MB-231–hERG1 and MDA-MB-231–hERG1-R531C cells.
  • References (5765)

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Citation: A. Becchetti, S. Crescioli, F. Zanieri, G. Petroni, R. Mercatelli, S. Coppola, L. Gasparoli, M. D'Amico, S. Pillozzi, O. Crociani, M. Stefanini, A. Fiore, L. Carraresi, V. Morello, S. Manoli, M. F. Brizzi, D. Ricci, M. Rinaldi, A. Masi, T. Schmidt, F. Quercioli, P. Defilippi, A. Arcangeli, The conformational state of hERG1 channels determines integrin association, downstream signaling, and cancer progression.. Sci. Signal. 10, eaaf3236 (2017).

© 2017 American Association for the Advancement of Science