Supplementary Materials

Supplementary Materials for:

Astrocyte-shed extracellular vesicles regulate the peripheral leukocyte response to inflammatory brain lesions

Alex M. Dickens, Luis B. Tovar-y-Romo, Seung-Wan Yoo, Amanda L. Trout, Mihyun Bae, Marlene Kanmogne, Bezawit Megra, Dionna W. Williams, Kennith W. Witwer, Mar Gacias, Nino Tabatadze, Robert N. Cole, Patrizia Casaccia, Joan W. Berman, Daniel C. Anthony, Norman J. Haughey*

*Corresponding author. Email: nhaughe1{at}jhmi.edu

This PDF file includes:

  • Fig. S1. Additional leukocyte, ceramide, and sphingomyelin measurements.
  • Fig. S2. Additional cytokine measurements and plasma EV counts after intrastriatal injection of IL-1β.
  • Fig. S3. EV isolation, quantitation, and liver cytokine response 24 hours after EV infusion in mice.
  • Fig. S4. IL-1β and TNFα promote formation of ceramide-rich membrane microdomains and EV release from astrocytes.
  • Fig. S5. EVs rapidly transverse endothelial cells in a BBB model system.
  • Fig. S6. Additional proteomic and ChIP results.
  • Legends for tables S1 and S2
  • Table S3. List of miRNA identified in exosomes and relative fold change.
  • Table S4. List of ChIP primers.
  • Legend for movie S1

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.02 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of all proteins identified in exosomes collected from control GFAP-GFP astrocytes.
  • Table S2 (Microsoft Excel format). List of all proteins identified in exosomes collected from IL-1β–stimulated GFAP-GFP astrocytes.
  • Movie S1 (.mov format). Visualization of IL-1β–induced release of EVs from cultured primary astrocytes.

[Download Tables S1 and S2]

[Download Movie S1]


Citation: A. M. Dickens, L. B. Tovar-y-Romo, S.-W. Yoo, A. L. Trout, M. Bae, M. Kanmogne, B. Megra, D. W. Williams, K. W. Witwer, M. Gacias, N. Tabatadze, R. N. Cole, P. Casaccia, J. W. Berman, D. C. Anthony, N. J. Haughey, Astrocyte-shed extracellular vesicles regulate the peripheral leukocyte response to inflammatory brain lesions.. Sci. Signal. 10, eaai7696 (2017).

© 2017 American Association for the Advancement of Science