Supplementary Materials

Supplementary Materials for:

YAP-mediated mechanotransduction determines the podocyte's response to damage

Markus M. Rinschen, Florian Grahammer, Ann-Kathrin Hoppe, Priyanka Kohli, Henning Hagmann, Oliver Kretz, Sabine Bertsch, Martin Höhne, Heike Göbe, Malte P. Bartram, Rajesh Kumar Gandhirajan, Marcus Krüger, Paul-Thomas Brinkkoetter, Tobias B. Huber, Martin Kann, Sara A. Wickström, Thomas Benzing,* Bernhard Schermer*

*Corresponding author. Email: thomas.benzing{at}uk-koeln.de (T.B.); bernhard.schermer{at}uk-koeln.de (B.S.)

This PDF file includes:

  • Fig. S1. Lack of mRNA regulation and YAP/TAZ stoichiometry determine the predominance of YAP for podocyte signaling in injury.
  • Fig. S2. Proteomic analysis of the PAN podocyte in vitro model demonstrates a global decrease in proteins translated from YAP/TAZ target genes.
  • Fig. S3. Time course analysis of PAN-induced proteomic changes in undifferentiated podocytes reveals a cluster of proteins with an extracellular matrix signature.
  • Fig. S4. The PAN-mediated decrease of YAP/TAZ target gene expression is concentration-dependent and can be observed in a mouse podocyte cell line.
  • Fig. S5. Differentiated podocytes demonstrate an inhibition of YAP signaling in response to PAN.
  • Fig. S6. YAP/TAZ is degraded in response to PAN in cultured cells.
  • Fig. S7. Growth and morphology of undifferentiated podocytes on soft and stiff matrices.
  • Fig. S8. Expression of the bona fide YAP target gene CTGF in cytochalasin-treated podocytes.
  • Fig. S9. Effect of YAP and YAPS112A overexpression on the podocyte proteome.
  • Fig. S10. Effect of PAN injury on mechanosignaling in vivo.
  • Fig. S11. Effect of podocyte-specific YAP increase in mice.
  • Fig. S12. Effects of VP on YAP and STAT target gene expression and on podocyte mechanosignaling.
  • Fig. S13. Validation of antibodies.
  • Table S1. List of all primary and secondary antibodies and dyes used in this study and the concentrations at which they were used.
  • Table S2. Primers used for qPCR.
  • Table S3. Primers for probe-based qPCR assays.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Proteomic analysis of PAN effect in rat glomeruli in Excel format.
  • Data file S2 (.txt format). Proteomic analysis of PAN effect in rat glomeruli in text format.
  • Data file S3 (Microsoft Excel format). Proteomic analysis of PAN effect in human podocytes in Excel format.
  • Data file S4 (.txt format). Proteomic analysis of PAN effect in human podocytes in text format.

[Download Data files S1 to S4]


Citation: M. M. Rinschen, F. Grahammer, A.-K. Hoppe, P. Kohli, H. Hagmann, O. Kretz, S. Bertsch, M. Höhne, H. Göbel, M. P. Bartram, R. K. Gandhirajan, M. Krüger, P.-T. Brinkkoetter, T. B. Huber, M. Kann, S. A. Wickström, T. Benzing, B. Schermer, YAP-mediated mechanotransduction determines the podocyte's response to damage. Sci. Signal. 10, eaaf8165 (2017).

© 2017 American Association for the Advancement of Science