Supplementary Materials
Supplementary Materials for:
The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB
Yukari Okita, Minori Kimura, Rudy Xie, Chen Chen, Larina Tzu-Wei Shen, Yurika Kojima, Hiroyuki Suzuki, Masafumi Muratani, Masao Saitoh, Kentaro Semba, Carl-Henrik Heldin, Mitsuyasu Kato*
*Corresponding author. Email: mit-kato{at}md.tsukuba.ac.jp
This PDF file includes:
- Fig. S1. Patients with MAFK-high breast tumors have shorter relapse-free survival rates.
- Fig. S2. Knockdown of MAFK reduces lung metastasis in 4T1 cells.
- Fig. S3. MAFK induces sphere formation, invasion, and EMT of NMuMG cells in culture.
- Fig. S4. MAFK induces the expression of multiple target genes.
- Fig. S5. Knockdown of MAFK or GPNMB suppresses sphere formation, migration, invasion, and EMT.
- Fig. S6. GPNMB activates cell migration and invasion but not monolayer culture cell proliferation.
- Fig. S7. MAFK or GPNMB knockdown in human breast cancer cell lines does not affect EMT phenotypes and cell migration.
- Fig. S8. SRC kinase inhibitor dasatinib suppresses sphere formation of human breast cancer cell lines in the presence of EGFR kinase inhibitor.
- Table S1. Primers for RT-PCR.
- Table S2. Primers for qPCR.
- Table S3. Primers for ChIP.
- Table S4. Data sets for prognosis analysis.
- References (58–63)
Technical Details
Format: Adobe Acrobat PDF
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Citation: Y. Okita, M. Kimura, R. Xie, C. Chen, L. T.-W. Shen, Y. Kojima, H. Suzuki, M. Muratani, M. Saitoh, K. Semba, C.-H. Heldin, M. Kato, The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal. 10, eaak9397 (2017).
