Supplementary Materials

Supplementary Materials for:

The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB

Yukari Okita, Minori Kimura, Rudy Xie, Chen Chen, Larina Tzu-Wei Shen, Yurika Kojima, Hiroyuki Suzuki, Masafumi Muratani, Masao Saitoh, Kentaro Semba, Carl-Henrik Heldin, Mitsuyasu Kato*

*Corresponding author. Email: mit-kato{at}md.tsukuba.ac.jp

This PDF file includes:

  • Fig. S1. Patients with MAFK-high breast tumors have shorter relapse-free survival rates.
  • Fig. S2. Knockdown of MAFK reduces lung metastasis in 4T1 cells.
  • Fig. S3. MAFK induces sphere formation, invasion, and EMT of NMuMG cells in culture.
  • Fig. S4. MAFK induces the expression of multiple target genes.
  • Fig. S5. Knockdown of MAFK or GPNMB suppresses sphere formation, migration, invasion, and EMT.
  • Fig. S6. GPNMB activates cell migration and invasion but not monolayer culture cell proliferation.
  • Fig. S7. MAFK or GPNMB knockdown in human breast cancer cell lines does not affect EMT phenotypes and cell migration.
  • Fig. S8. SRC kinase inhibitor dasatinib suppresses sphere formation of human breast cancer cell lines in the presence of EGFR kinase inhibitor.
  • Table S1. Primers for RT-PCR.
  • Table S2. Primers for qPCR.
  • Table S3. Primers for ChIP.
  • Table S4. Data sets for prognosis analysis.
  • References (5863)

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Citation: Y. Okita, M. Kimura, R. Xie, C. Chen, L. T.-W. Shen, Y. Kojima, H. Suzuki, M. Muratani, M. Saitoh, K. Semba, C.-H. Heldin, M. Kato, The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal. 10, eaak9397 (2017).

© 2017 American Association for the Advancement of Science