Supplementary Materials

Supplementary Materials for:

Unphosphorylated ISGF3 drives constitutive expression of interferon-stimulated genes to protect against viral infections

Wenshi Wang, Yuebang Yin, Lei Xu, Junhong Su, Fen Huang, Yijin Wang, Patrick P. C. Boor, Kan Chen, Wenhui Wang, Wanlu Cao, Xinying Zhou, Pengyu Liu, Luc J. W. van der Laan, Jaap Kwekkeboom, Maikel P. Peppelenbosch, Qiuwei Pan*

*Corresponding author. Email: q.pan{at}erasmusmc.nl

This PDF file includes:

  • Fig. S1. Cells sustain basal ISG expression under homeostatic conditions.
  • Fig. S2. Sustained basal ISG expression is independent of IFN production.
  • Fig. S3. STAT1, STAT2, and IRF9 are required for constitutive ISG expression.
  • Fig. S4. The overexpression of U-ISGF3 leads to increased ISG expression.
  • Fig. S5. U-ISGF3 stimulates ISG expression independently of IFN production.
  • Fig. S6. IPCs produce IFNs to activate the JAK-STAT pathway, thus stimulating ISG expression and antiviral activity.
  • Fig. S7. U-ISGF3 stimulates ISG expression independently of upstream elements of the IFN signaling pathway.
  • Table S1. Sequences of the shRNAs used in the study.
  • Table S2. Sequences of the primers used for the qRT-PCR analysis of human cells.
  • Table S3. Sequences of the primers used for the qRT-PCR analysis of mouse cells.

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Citation: W. Wang, Y. Yin, L. Xu, J. Su, F. Huang, Y. Wang, P. P. C. Boor, K. Chen, W. Wang, W. Cao, X. Zhou, P. Liu, L. J. W. van der Laan, J. Kwekkeboom, M. P. Peppelenbosch, Q. Pan, Unphosphorylated ISGF3 drives constitutive expression of interferon-stimulated genes to protect against viral infections. Sci. Signal. 10, eaah4248 (2017).

© 2017 American Association for the Advancement of Science