Supplementary Materials

Supplementary Materials for:

Genome-wide identification and characterization of Notch transcription complex–binding sequence-paired sites in leukemia cells

Eric Severson, Kelly L. Arnett, Hongfang Wang, Chongzhi Zang, Len Taing, Hudan Liu, Warren S. Pear, X. Shirley Liu, Stephen C. Blacklow,* Jon C. Aster*

*Corresponding author. Email: stephen_blacklow{at}hms.harvard.edu (S.C.B.); jaster{at}partners.org (J.C.A.)

This PDF file includes:

  • Fig. S1. Characterization of the dimerization-defective NOTCH1 mutant R1984A in FRET, reporter gene, and local ChIP assays.
  • Fig. S2. Sequences used to assess the effect of spacer length on NTC dimerization.
  • Fig. S3. Endogenous murine Hes5 promoter and Notch-dependent Myc enhancer SPSs support NTC dimerization in FRET assays.
  • Fig. S4. RBPJ binding affinity for DNA correlates with FRET efficiency.
  • Fig. S5. Identification of SPSs and Notch dimer–dependent target genes in murine T6E T-ALL cells.
  • Fig. S6. HES5 and HANR1 expression depend on NTC dimerization.
  • Fig. S7. CRISPR/Cas9 targeting of RBPJ/NOTCH1 binding sites in the E1 and E2 elements 5′ of HES5.
  • Fig. S8. Chromatin landscapes near HES5 in T-ALL cell lines.
  • Fig. S9. Coregulation of IGF1R and LUNAR1 by an intronic IGF1R SPS.
  • Table S3. Primers.
  • Legends for tables S1 and S2
  • Reference (47)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Dynamic NOTCH1 binding sites in the genome of CUTLL1 T-ALL cells.
  • Table S2 (Microsoft Excel format). Notch-sensitive genes in human CUTLL1 T-ALL cells.

Citation: E. Severson, K. L. Arnett, H. Wang, C. Zang, L. Taing, H. Liu, W. S. Pear, X. S. Liu, S. C. Blacklow, J. C. Aster, Genome-wide identification and characterization of Notch transcription complex–binding sequence-paired sites in leukemia cells. Sci. Signal. 10, eaag1598 (2017).

© 2017 American Association for the Advancement of Science