Supplementary Materials

Supplementary Materials for:

BMP8A sustains spermatogenesis by activating both SMAD1/5/8 and SMAD2/3 in spermatogonia

Fang-Ju Wu, Ting-Yu Lin, Li-Ying Sung, Wei-Fang Chang, Po-Chih Wu, Ching-Wei Luo*

*Corresponding author. Email: cwluo{at}ym.edu.tw

This PDF file includes:

  • Fig. S1. Schematic representation, expression, and activity tests of BMP-expressing constructs.
  • Fig. S2. Knockdown of ALK3, ALK4, ALK5, or ALK6 suppresses reporter activity stimulated by BMP8A.
  • Fig. S3. Interaction between BMP8A and TGFBR2, ALK3, or ALK5.
  • Fig. S4. Localization of BMP8 in the neonatal mouse testis.
  • Fig. S5. Receptor knockdown efficiency in spermatogonia in vitro.
  • Fig. S6. BMP8A treatment increases the numbers of haploid cells in cultured testis explants.
  • Table S1. Fertilization and development of zygotes after ROSI.
  • Table S2. Primers used for qPCR.

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Citation: F.-J. Wu, T.-Y. Lin, L.-Y. Sung, W.-F. Chang, P.-C. Wu, C.-W. Luo, BMP8A sustains spermatogenesis by activating both SMAD1/5/8 and SMAD2/3 in spermatogonia. Sci. Signal. 10, eaal1910 (2017).

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