Supplementary Materials

Supplementary Materials for:

The cytotoxic type 3 secretion system 1 of Vibrio rewires host gene expression to subvert cell death and activate cell survival pathways

Nicole J. De Nisco, Mohammed Kanchwala, Peng Li, Jessie Fernandez, Chao Xing, Kim Orth*

*Corresponding author. Email: kim.orth{at}utsouthwestern.edu

This PDF file includes:

  • Fig. S1. Confocal microscopy of POR4:T3SS1-infected fibroblasts.
  • Fig. S2. Principal components analysis of RNA-seq data.
  • Fig. S3. qRT-PCR analysis of cytokines in HeLa cells and RAW264.7 macrophages.
  • Fig. S4. qRT-PCR analysis of top fibroblast POR4 versus POR3 differentially expressed genes in HeLa cells and RAW264.7 macrophages.
  • Fig. S5. U0126 treatment inhibits POR3-induced Rhob expression in RAW264.7 macrophages.
  • Legends for tables S1 to S6
  • Table S7. Primer list.

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Technical Details

Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Significantly differentially expressed genes in uninfected versus POR4:T3SS1-infected fibroblasts.
  • Table S2 (Microsoft Excel format). Significantly differentially expressed genes in uninfected versus POR3:T3SS1+-infected fibroblasts.
  • Table S3 (Microsoft Excel format). Significantly differentially expressed genes in POR4:T3SS1- versus POR3:T3SS1+-infected fibroblasts.
  • Table S4 (Microsoft Excel format). IPA canonical pathways uninfected versus POR4:T3SS1.
  • Table S5 (Microsoft Excel format). IPA canonical pathways uninfected versus POR3:T3SS1+.
  • Table S6 (Microsoft Excel format). IPA network analysis (disease and biological functions) of uninfected versus POR3:T3SS1+.

[Download Tables S1 to S6]


Citation: N. J. De Nisco, M. Kanchwala, P. Li, J. Fernandez, C. Xing, K. Orth, The cytotoxic type 3 secretion system 1 of Vibrio rewires host gene expression to subvert cell death and activate cell survival pathways. Sci. Signal. 10, eaal4501 (2017).

© 2017 American Association for the Advancement of Science