Supplementary Materials

Supplementary Materials for:

Nrf2 inactivation enhances placental angiogenesis in a preeclampsia mouse model and improves maternal and fetal outcomes

Masahiro Nezu, Tomokazu Souma, Lei Yu, Hiroki Sekine, Nobuyuki Takahashi, Andrew Zu-Sern Wei, Sadayoshi Ito, Akiyoshi Fukamizu, Zsuzsanna K. Zsengeller, Tomohiro Nakamura, Atsushi Hozawa, S. Ananth Karumanchi, Norio Suzuki,* Masayuki Yamamoto*

*Corresponding author. Email: sunorio{at}med.tohoku.ac.jp (N.S.); masiyamamoto{at}med.tohoku.ac.jp (M.Y.)

This PDF file includes:

  • Fig. S1. Mouse models used in this study.
  • Fig. S2. Fetal body weight, systolic blood pressure, and gene expression profiles of RAS components in NCP and PAH mice with Keap1 or Nrf2 genetic mutation.
  • Fig. S3. Genetic deletion of Nrf2 improves vascular plexus selectively in the PAH placenta.
  • Fig. S4. Expression profiles of Nrf2 target genes in the placenta of PAH mice.
  • Fig. S5. Microarray analysis to identify an Nrf2-dependent PAH-specific gene signature.
  • Fig. S6. Genetic modification of the Keap1-Nrf2 system in PAH mice does not significantly change the plasma concentrations of sFLT1 and PLGF2.
  • Fig. S7. Induction of Nrf2 target gene expression in the labyrinth zone of PAH-WT mice by pharmacological activation of Nrf2.
  • Table S1. Primer sequences for genotyping mouse lines.
  • Table S2. Primer sequences for qRT-PCR analysis.
  • Legend for data file S1

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Microarray data revealed candidate genes for an Nrf2-dependent PAH-specific gene signature.

Citation: M. Nezu, T. Souma, L. Yu, H. Sekine, N. Takahashi, A. Z.-S. Wei, S. Ito, A. Fukamizu, Z. K. Zsengeller, T. Nakamura, A. Hozawa, S. A. Karumanchi, N. Suzuki, M. Yamamoto, Nrf2 inactivation enhances placental angiogenesis in a preeclampsia mouse model and improves maternal and fetal outcomes. Sci. Signal. 10, eaam5711 (2017).

© 2017 American Association for the Advancement of Science