Supplementary Materials

Supplementary Materials for:

Key determinants of selective binding and activation by the monocyte chemoattractant proteins at the chemokine receptor CCR2

Zil E. Huma, Julie Sanchez, Herman D. Lim, Jessica L. Bridgford, Cheng Huang, Bradyn J. Parker, Jiann G. Pazhamalil, Benjamin T. Porebski, Kevin D. G. Pfleger, J. Robert Lane, Meritxell Canals,* Martin J. Stone*

*Corresponding author. Email: martin.stone{at}monash.edu (M.J.S.); meri.canals{at}monash.edu (M.C.)

This PDF file includes:

  • Fig. S1. Neither MCP-2 nor MCP-3 is a biased agonist at CCR2 relative to MCP-1.
  • Fig. S2. NMR spectra of chemokine chimeras.
  • Fig. S3. MCP3-111 displays biased agonism relative to MCP-3.
  • Fig. S4. Homology model of CCR2 bound to MCP-1, showing the positions of the mutated residues.
  • Fig. S5. 125I–MCP-1 competition binding and ERK1/2 phosphorylation concentration response curves for CCR2 mutants.
  • Fig. S6. Graphical comparisons of chemokine binding and ERK1/2 phosphorylation parameters across the set of CCR2 mutants.
  • Fig. S7. The amino acid sequences of MCP-1 (P8A), WT MCP-3, and the chimeric chemokines.
  • Table S1. Biased agonism parameters for MCP chemokines at CCR2.
  • Table S2. MCP3-111 displays biased agonism at CCR2 compared to MCP-3.
  • Table S3. Expected and observed molecular masses of WT and chimeric chemokines.

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Citation: Z. E. Huma, J. Sanchez, H. D. Lim, J. L. Bridgford, C. Huang, B. J. Parker, J. G. Pazhamalil, B. T. Porebski, K. D. G. Pfleger, J. R. Lane, M. Canals, M. J. Stone, Key determinants of selective binding and activation by the monocyte chemoattractant proteins at the chemokine receptor CCR2. Sci. Signal. 10, eaai8529 (2017).

© 2017 American Association for the Advancement of Science