Supplementary Materials

Supplementary Materials for:

A disease-linked ULBP6 polymorphism inhibits NKG2D-mediated target cell killing by enhancing the stability of NKG2D ligand binding

Jianmin Zuo, Carrie R. Willcox, Fiyaz Mohammed, Martin Davey, Stuart Hunter, Kabir Khan, Ayman Antoun, Poonam Katakia, Joanne Croudace, Charlotte Inman, Helen Parry, David Briggs, Ram Malladi, Benjamin E. Willcox,* Paul Moss*

*Corresponding author. Email: p.moss{at}bham.ac.uk (P.M.); b.willcox{at}bham.ac.uk (B.E.W.)

This PDF file includes:

  • Fig. S1. Validation of the specificity of the ULBP2, ULBP5, and ULBP6 qRT-PCR assays.
  • Fig. S2. SPR analysis of NKG2D-ULBP interactions.
  • Fig. S3. Structural characteristics of the NKG2D-ULBP0602 complex.
  • Fig. S4. Stabilization of the NKG2D-ULBP3 complex interface.
  • Fig. S5. Sequence alignment of the ULBP family members.
  • Fig. S6. ULBP0601 elicits stronger cytotoxicity than does ULBP0602 in a 51Cr-based assay.
  • Fig. S7. Analysis of the cytotoxicity of NKL cells to Jurkat-ULBP0601 and Jurkat-ULBP0602 cells.
  • Fig. S8. NK cells captured by ULBP0602 inhibit the killing of ULBP0601-expressing target cells.
  • Fig. S9. Analysis of the effects of different amounts of ULBP0601 and ULBP0602 on NK cell activation.
  • Table S1. Comparison of the affinities of NKG2D for different NKG2D ligands.
  • Table S2. Data processing and refinement statistics for the NKGD2-ULBP0602 complex.
  • Table S3. Interactions mediated by polar residues at the NKGD2-ULBP6 interface.
  • Table S4. Comparison of the hydrophobic and van der Waal’s contacts that stabilize the NKG2D-ULBP0602 and NKG2D-ULBP3 interfaces.

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Citation: J. Zuo, C. R. Willcox, F. Mohammed, M. Davey, S. Hunter, K. Khan, A. Antoun, P. Katakia, J. Croudace, C. Inman, H. Parry, D. Briggs, R. Malladi, B. E. Willcox, P. Moss, A disease-linked ULBP6 polymorphism inhibits NKG2D-mediated target cell killing by enhancing the stability of NKG2D ligand binding. Sci. Signal. 10, eaai8904 (2017).

© 2017 American Association for the Advancement of Science