Supplementary Materials for:

T-plastin is essential for basement membrane assembly and epidermal morphogenesis

Eyal Dor-On, Shaul Raviv, Yonatan Cohen, Orit Adir, Krishnanand Padmanabhan, Chen Luxenburg*

*Corresponding author. Email: lux{at}post.tau.ac.il

This PDF file includes:

• Fig. S1. Pls3 KD by shRNAs Pls3-353 and Pls3-1215 causes defects analogous to those induced by Pls3-2958.
• Fig. S2. Pls3 KD does not induce ectopic expression of K6 or apoptosis.
• Fig. S3. Pls3 KD does not alter the distribution of desmoplakin or ZO1.
• Fig. S4. Defects in PAR3 distribution cannot explain the BM phenotype in Pls3-depleted epidermis.
• Fig. S5. Pls3 does not affect expression of cytoskeleton, adhesion, and BM genes or activation of β1 integrin.
• Fig. S6. Actb KD in small clones by shRNA Actb-1652 causes defects analogous to those induced by Actb-289.
• Fig. S7. Pls3 KD by shRNA Pls3-353 causes myosin II defects analogous to those induced by Pls3-2958.
• Fig. S8. Full sized H2B-GFP images from Figs. 1 to 5.
• Table S1. RT-qPCR primers.

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Citation: E. Dor-On, S. Raviv, Y. Cohen, O. Adir, K. Padmanabhan, C. Luxenburg, T-plastin is essential for basement membrane assembly and epidermal morphogenesis. Sci. Signal. 10, eaal3154 (2017).

© 2017 American Association for the Advancement of Science