Supplementary Materials

Supplementary Materials for:

Radiation inhibits salivary gland function by promoting STIM1 cleavage by caspase-3 and loss of SOCE through a TRPM2-dependent pathway

Xibao Liu, Baijuan Gong, Lorena Brito de Souza, Hwei Ling Ong, Krishna P. Subedi, Kwong Tai Cheng, William Swaim, Changyu Zheng, Yasuo Mori, Indu S. Ambudkar*

*Corresponding author. Email: indu.ambudkar{at}nih.gov

This PDF file includes:

  • Fig. S1. Effect of irradiation on SOCE in dispersed salivary gland acinar cells from TRPM2+/+ and TRPM2−/− mice and HSG cells.
  • Fig. S2. H2O2-induced changes in [Ca2+]mt in dispersed salivary gland acinar cells from TRPM2+/+ and TRPM2−/− mice and role of MCU in enhancement of [Ca2+]mt.
  • Fig. S3. H2O2-induced changes in mitochondrial ROS in dispersed salivary gland acinar cells from TRPM2+/+ and TRPM2−/− mice and effect of MitoTEMPO on [Ca2+]mt and mitochondrial ROS.
  • Fig. S4. STIM1 cleavage in staurosporine- and H2O2-treated cells is mediated by caspase-3.
  • Fig. S5. Knockdown of caspase-3 protects against radiation-induced loss of SOCE.

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Citation: X. Liu, B. Gong, L. B. de Souza, H. L. Ong, K. P. Subedi, K. T. Cheng, W. Swaim, C. Zheng, Y. Mori, I. S. Ambudkar, Radiation inhibits salivary gland function by promoting STIM1 cleavage by caspase-3 and loss of SOCE through a TRPM2-dependent pathway. Sci. Signal. 10, eaal4064 (2017).

© 2017 American Association for the Advancement of Science