Supplementary Materials

Supplementary Materials for:

Ligand-activated Notch undergoes DTX4-mediated ubiquitylation and bilateral endocytosis before ADAM10 processing

Patricia Chastagner, Eric Rubinstein, Christel Brou*

*Corresponding author. Email: christel.brou{at}pasteur.fr

This PDF file includes:

  • Fig. S1. Diagram of the constructs used in this study.
  • Fig. S2. Characterization of the cell lines used in this study.
  • Fig. S3. Effect of DTX silencing and dynamin K44A overexpression on transendocytosis.
  • Fig. S4. Specificity of GI on ADAM-mediated cleavage of N1.
  • Fig. S5. Clustering of N1 with DTX4 at the cell surface upon Dll-Fc activation.
  • Fig. S6. Ubiquitylation of N1 upon Dll-Fc–mediated activation.
  • Fig. S7. Colocalization of N1 and ADAM10 upon Dll-Fc–mediated activation.
  • Legends for movies S1 to S7

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.80 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). DTX4-GFP in unstimulated cells.
  • Movie S2 (.mov format). The first 40 min of N1 activation.
  • Movie S3 (.mov format). N1 in moving vesicles after 60 min of activation.
  • Movie S4 (.mov format). Tracking N1 from the cell surface into fast-moving vesicles.
  • Movie S5 (.mov format). N1 in moving vesicles after 60 min of activation in the presence of DAPT.
  • Movie S6 (.mov format). Notch in moving vesicles after 60 min of activation in the presence of GI.
  • Movie S7 (.mov format). DTX4-GFP in stimulated cells.

Citation: P. Chastagner, E. Rubinstein, C. Brou, Ligand-activated Notch undergoes DTX4-mediated ubiquitylation and bilateral endocytosis before ADAM10 processing. Sci. Signal. 10, eaag2989 (2017).

© 2017 American Association for the Advancement of Science