Supplementary Materials

Supplementary Materials for:

Voltage-dependent Ca2+ entry into smooth muscle during contraction promotes endothelium-mediated feedback vasodilation in arterioles

Christopher J. Garland, Pooneh Bagher, Chloe Powell, Xi Ye, Hamish A.L. Lemmey, Lyudmyla Borysova, Kim A. Dora*

*Corresponding author. Email: kim.dora{at}pharm.ox.ac.uk

This PDF file includes:

  • Fig. S1. Myogenic tone relies on L-type VDCCs in arterioles.
  • Fig. S2. Methods for analyzing endothelial cell Ca2+ events in cannulated arterioles.
  • Fig. S3. Block of arteriole constriction using blebbistatin.
  • Fig. S4. Characteristics of arteriolar endothelial cell Ca2+ events.
  • Fig. S5. Profile of Ca2+ release pathways in arteriolar endothelial cells.
  • Fig. S6. Loading heparin into arteriolar endothelial cells inhibits IP3Rs.
  • Fig. S7. Dichotomous effects of 2-APB.
  • Fig. S8. Observations on the use of cell-permeant inhibitors of phospholipase C and IP3Rs.
  • Fig. S9. Effect of ryanodine on arteriolar Ca2+ events.
  • Fig. S10. Effect of nifedipine on vasoconstriction to phenylephrine.
  • Fig. S11. Simultaneous imaging of endothelial and smooth muscle cell Ca2+ events at the arteriolar midplane.
  • Fig. S12. Characteristics of arteriole vasoconstriction to phenylephrine.
  • Fig. S13. Intercellular Ca2+ circuit in skeletal muscle arterioles.
  • Table S1. Effect of nifedipine on vasoconstriction to BayK and KCl.
  • Table S2. Details of rat genes detected with TaqMan probes.
  • Legends for movies S1 to S6

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Time course of endothelial cell Ca2+ events in response to BayK in a pressurized arteriole.
  • Movie S2 (.mov format). Time course of endothelial cell Ca2+ events in response to BayK in an isolated tube.
  • Movie S3 (.mov format). Time course of endothelial cell Ca2+ events in response to phenylephrine in an isolated tube.
  • Movie S4 (.mov format). Time course of endothelial cell Ca2+ events in response to KCl in a pressurized arteriole.
  • Movie S5 (.mov format). Time course of endothelial cell Ca2+ events in response to 4-aminopyridine in a pressurized arteriole.
  • Movie S6 (.mov format). Time course of endothelial cell Ca2+ events in response to phenylephrine in a pressurized arteriole.

Citation: C. J. Garland, P. Bagher, C. Powell, X. Ye, H. A.L. Lemmey, L. Borysova, K. A. Dora, Voltage-dependent Ca2+ entry into smooth muscle during contraction promotes endotheliummediated feedback vasodilation in arterioles. Sci. Signal. 10, eaal3806 (2017).

© 2017 American Association for the Advancement of Science