Supplementary Materials for:

Voltage-dependent Ca²⁺ entry into smooth muscle during contraction promotes endothelium-mediated feedback vasodilation in arterioles

Christopher J. Garland, Pooneh Bagher, Chloe Powell, Xi Ye, Hamish A.L. Lemmey, Lyudmyla Borysova, Kim A. Dora*

*Corresponding author. Email: kim.dora{at}pharm.ox.ac.uk

This PDF file includes:

• Fig. S1. Myogenic tone relies on L-type VDCCs in arterioles.
• Fig. S2. Methods for analyzing endothelial cell Ca²⁺ events in cannulated arterioles.
• Fig. S3. Block of arteriole constriction using blebbistatin.
• Fig. S4. Characteristics of arteriolar endothelial cell Ca²⁺ events.
• Fig. S5. Profile of Ca²⁺ release pathways in arteriolar endothelial cells.
• Fig. S6. Loading heparin into arteriolar endothelial cells inhibits IP₃Rs.
• Fig. S7. Dichotomous effects of 2-APB.
• Fig. S8. Observations on the use of cell-permeant inhibitors of phospholipase C and IP₃Rs.
• Fig. S9. Effect of ryanodine on arteriolar Ca²⁺ events.
• Fig. S10. Effect of nifedipine on vasoconstriction to phenylephrine.
• Fig. S11. Simultaneous imaging of endothelial and smooth muscle cell Ca²⁺ events at the arteriolar midplane.
• Fig. S12. Characteristics of arteriole vasoconstriction to phenylephrine.
• Fig. S13. Intercellular Ca²⁺ circuit in skeletal muscle arterioles.
• Table S1. Effect of nifedipine on vasoconstriction to BayK and KCl.
• Table S2. Details of rat genes detected with TaqMan probes.
• Legends for movies S1 to S6

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mov format). Time course of endothelial cell Ca²⁺ events in response to BayK in a pressurized arteriole.
• Movie S2 (.mov format). Time course of endothelial cell Ca²⁺ events in response to BayK in an isolated tube.
• Movie S3 (.mov format). Time course of endothelial cell Ca²⁺ events in response to phenylephrine in an isolated tube.
• Movie S4 (.mov format). Time course of endothelial cell Ca²⁺ events in response to KCl in a pressurized arteriole.
• Movie S5 (.mov format). Time course of endothelial cell Ca²⁺ events in response to 4-aminopyridine in a pressurized arteriole.
• Movie S6 (.mov format). Time course of endothelial cell Ca²⁺ events in response to phenylephrine in a pressurized arteriole.

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Citation: C. J. Garland, P. Bagher, C. Powell, X. Ye, H. A.L. Lemmey, L. Borysova, K. A. Dora, Voltage-dependent Ca²⁺ entry into smooth muscle during contraction promotes endotheliummediated feedback vasodilation in arterioles. Sci. Signal. 10, eaal3806 (2017).

© 2017 American Association for the Advancement of Science