Supplementary Materials

Supplementary Materials for:

Gene expression profiles of brain endothelial cells during embryonic development at bulk and single-cell levels

Mike Hupe,* Minerva Xueting Li, Susanne Kneitz, Daria Davydova, Chika Yokota, Julianna Kele, Belma Hot, Jan M. Stenman, Manfred Gessler

*Corresponding author. Email: mike.hupe{at}uni-wuerzburg.de

This PDF file includes:

  • Fig. S1. Gene expression in the embryonic CNS vasculature at E14.5.
  • Fig. S2. Translated transcripts encoding transporters in the embryonic CNS vasculature.
  • Fig. S3. Translated transcripts encoding molecular markers related to physical barrier properties in the embryonic CNS vasculature.
  • Fig. S4. Translated transcripts encoding transcription factors in embryonic vasculature.
  • Fig. S5. Cdh5CreERT2 driver line activity is not strictly limited to ECs only.
  • Fig. S6. Validation of TRAP-seq results by in situ hybridization.
  • Fig. S7. Brain EC maturation during embryonic development.
  • Fig. S8. Ctnnb1 deletion–dependent molecular changes in brain ECs.
  • Fig. S9. Single-cell sequence reads and mapping.
  • Fig. S10. High EC heterogeneity at the single-cell level for genes encoding general EC markers or mCherry.
  • Fig. S11. High EC heterogeneity at the single-cell level for genes encoding specific BBB markers.
  • Fig. S12. High level of EC heterogeneity.
  • Fig. S13. High EC heterogeneity at the single-cell level for genes encoding transcription factors identified as enriched in CNS vasculature by TRAP-seq.
  • Legends for tables S1 to S28

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Specifically translated genes in brain ECs.
  • Table S2 (Microsoft Excel format). Brain EC–enriched transcripts.
  • Table S3 (Microsoft Excel format). Transcripts shared between ECs from all other organs.
  • Table S4 (Microsoft Excel format). Brain EC–depleted transcripts.
  • Table S5 (Microsoft Excel format). Specifically translated genes in brain ECs during embryonic development.
  • Table S6 (Microsoft Excel format). Regulated transcripts in brain ECs during embryonic development.
  • Table S7 (Microsoft Excel format). Transcripts with increasing translation in brain ECs during embryonic development.
  • Table S8 (Microsoft Excel format). Transcripts with decreasing translation in brain ECs during embryonic development.
  • Table S9 (Microsoft Excel format). Genes with increasing and subsequently decreasing translation in brain ECs during embryonic development.
  • Table S10 (Microsoft Excel format). Genes with decreasing and subsequently increasing translation in brain ECs during embryonic development.
  • Table S11 (Microsoft Excel format). E14.5 brain EC–enriched transcripts during embryonic development.
  • Table S12 (Microsoft Excel format). E14.5 shared EC transcripts during embryonic development.
  • Table S13 (Microsoft Excel format). E14.5 brain EC–depleted genes during embryonic development.
  • Table S14 (Microsoft Excel format). Genes encoding angiogenesis marker in brain ECs during embryonic development.
  • Table S15 (Microsoft Excel format). Genes encoding proliferation and cell cycle marker in brain ECs during embryonic development.
  • Table S16 (Microsoft Excel format). Ctnnb1 KO–deleted transcripts in brain ECs at E14.5.
  • Table S17 (Microsoft Excel format). Ctnnb1 KO–deleted transcripts in brain ECs at E17.5.
  • Table S18 (Microsoft Excel format). E14.5 brain EC–enriched transcripts in Ctnnb1 KO brain ECs.
  • Table S19 (Microsoft Excel format). Ctnnb1 KO–enriched transcripts in brain ECs at E14.5.
  • Table S20 (Microsoft Excel format). Ctnnb1 KO–enriched transcripts in brain ECs at E17.5.
  • Table S21 (Microsoft Excel format). E14.5 shared EC transcripts in Ctnnb1 KO brain ECs.
  • Table S22 (Microsoft Excel format). Genes encoding angiogenesis marker in Ctnnb1 KO brain ECs.
  • Table S23 (Microsoft Excel format). E14.5 brain EC–depleted genes in Ctnnb1 KO brain ECs.
  • Table S24 (Microsoft Excel format). Genes encoding proliferation and cell cycle marker in Ctnnb1 KO brain ECs.
  • Table S25 (Microsoft Excel format). Genes encoding housekeeping markers in single cells.
  • Table S26 (Microsoft Excel format). Genes encoding EC markers in single cells.
  • Table S27 (Microsoft Excel format). Genes encoding neuronal markers in single cells.
  • Table S28 (Microsoft Excel format). Genes encoding housekeeping markers in single HEK293T cells.

[Download Tables S1 to S28]


Citation: M. Hupe, M. X. Li, S. Kneitz, D. Davydova, C. Yokota, J. Kele, B. Hot, J. M. Stenman, M. Gessler, Gene expression profiles of brain endothelial cells during embryonic development at bulk and single-cell levels. Sci. Signal. 10, eaag2476 (2017).

© 2017 American Association for the Advancement of Science