Supplementary Materials

Supplementary Materials for:

TRIF-dependent Toll-like receptor signaling suppresses Scd1 transcription in hepatocytes and prevents diet-induced hepatic steatosis

Jing Chen, Jin Li, Jensen H. C. Yiu, Jenny K. W. Lam, Chi-Ming Wong, Bernhard Dorweiler, Aimin Xu,* Connie W. Woo*

*Corresponding author. Email: cwhwoo{at}hku.hk (C.W.W.); amxu{at}hku.hk (A.X.)

This PDF file includes:

  • Fig. S1. Trif−/− mice have similar body weight and fat mass as wild-type mice but increased fasting blood glucose after HFD feeding.
  • Fig. S2. Hepatic inflammation, serum insulin concentrations, and serum lipids were similar between HFD-fed wild-type and Trif−/− mice.
  • Fig. S3. HFD did not increase CHOP abundance or apoptosis in wild-type and Trif−/− mice.
  • Fig. S4. Lipogenic gene expression did not increase in wild-type and Trif−/− mice fed an NCD.
  • Fig. S5. Poly(I:C)–stimulated phosphorylation of IRF3 and suppressed palmitic acid–induced Scd1 expression in hepatocytes in a TLR3-dependent manner.
  • Fig. S6. RNA was detected in the serum of HFD-fed mice and taken up by hepatocytes.
  • Fig. S7. Lv-SCD1-siRNA decreased SCD1 abundance.
  • Fig. S8. Activation of TRIF by poly(I:C) decreased fasting blood glucose concentrations in HFD-fed wild-type mice without significantly affecting body weight, fat mass, or hepatic inflammation.
  • Table S1. IRF3 binding motifs in the Scd1 promoter in different species.
  • Table S2. Primer sequences for specific genes.

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Citation: J. Chen, J. Li, J. H. C. Yiu, J. K. W. Lam, C.-M. Wong, B. Dorweiler, A. Xu, C. W. Woo, TRIF-dependent Toll-like receptor signaling suppresses Scd1 transcription in hepatocytes and prevents diet-induced hepatic steatosis. Sci. Signal. 10, eaal3336 (2017).

© 2017 American Association for the Advancement of Science