Supplementary Materials

Supplementary Materials for:

Loss of SPDEF and gain of TGFBI activity after androgen deprivation therapy promote EMT and bone metastasis of prostate cancer

Wei-Yu Chen, Yuan-Chin Tsai, Hsiu-Lien Yeh, Florent Suau, Kuo-Ching Jiang, Ai-Ning Shao, Jiaoti Huang, Yen-Nien Liu*

*Corresponding author. Email: liuy{at}tmu.edu.tw

This PDF file includes:

  • Fig. S1. Induction of TGFBI is involved in the activated EMT, activated TGFβ signaling, and inactivated AR signaling signature in prostate cancer.
  • Fig. S2. The effect of TGFBI in AR-positive prostate cancer cells.
  • Fig. S3. Induction of TGFBI is associated with decreased androgen-activated SPDEF expression in prostate cancer patient samples.
  • Fig. S4. Role of TGFBI in EMT after SPDEF knockdown in AR-inhibited prostate cancer cells.
  • Table S1. Primer sequences of the promoter reporter constructs.
  • Table S2. Real-time qPCR primer sequences.
  • Table S3. Western blotting antibodies.
  • Table S4. ChIP antibodies and primer sequences.
  • Table S5. IHC staining antibodies.

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Citation: W.-Y. Chen, Y.-C. Tsai, H.-L. Yeh, F. Suau, K.-C. Jiang, A.-N. Shao, J. Huang, Y.-N. Liu, Loss of SPDEF and gain of TGFBI activity after androgen deprivation therapy promote EMT and bone metastasis of prostate cancer. Sci. Signal. 10, eaam6826 (2017).

© 2017 American Association for the Advancement of Science