Supplementary Materials for:

Multiplex quantitative assays indicate a need for re-evaluating reported small-molecule TrkB agonists

Umed Boltaev, Yves Meyer, Farangis Tolibzoda, Teresa Jacques, Madalee Gassaway, Qihong Xu, Florence Wagner, Yan-Ling Zhang, Michelle Palmer, Edward Holson, Dalibor Sames*

*Corresponding author. Email: sames{at}chem.columbia.edu

This PDF file includes:

• Fig. S1. Antibody screen for sandwich ELISA.
• Fig. S2. Characterization of assays in TrkB-expressing cells.
• Fig. S3. Characterization of reported agonists in TrkB-expressing cells and HTS results.
• Fig. S4. Western blot analysis of reported agonists in HEK-TrkB cells.
• Fig. S5. Western blot analysis of reported agonists in cortical neuron culture.
• Fig. S6. Activity of 7,8-DHF and LM22A-4 in cortical neuron culture under different conditions.
• Fig. S7. K252a inhibition of ERK1/2 in cortical neuron culture.
• Fig. S8. K252a inhibition in CellSensor assay.
• Table S1. S/B values of ELISA and ELFI in cortical neuron culture.
• Table S2. EC₅₀ values of neurotrophic factors.
• Table S3. List of antibodies used in this study.
• Reference (66)

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Citation: U. Boltaev, Y. Meyer, F. Tolibzoda, T. Jacques, M. Gassaway, Q. Xu, F. Wagner, Y.-L. Zhang, M. Palmer, E. Holson, D. Sames, Multiplex quantitative assays indicate a need for re-evaluating reported small-molecule TrkB agonists. Sci. Signal. 10, eaal1670 (2017).

© 2017 American Association for the Advancement of Science