Supplementary Materials

Supplementary Materials for:

Blockade of surface-bound TGF-β on regulatory T cells abrogates suppression of effector T cell function in the tumor microenvironment

Sadna Budhu, David A. Schaer, Yongbiao Li, Ricardo Toledo-Crow, Katherine Panageas, Xia Yang, Hong Zhong, Alan N. Houghton, Samuel C. Silverstein, Taha Merghoub,* Jedd D. Wolchok*

*Corresponding author. Email: merghout{at}mskcc.org (T.M.); wolchokj{at}mskcc.org (J.D.W.)

This PDF file includes:

  • Summary of statistical analyses
  • Fig. S1. Analysis of immune cell infiltrates in tumors from DT-treated Foxp3-DTR mice.
  • Fig. S2. In vivo depletion of CCR2+ cells in CCR2-DTR mice has no effect on the suppression of CD8+ T cell–mediated killing by the tumor microenvironment.
  • Fig. S3. Depleting Tregs ex vivo with anti-CD25 MicroBeads has no effect on the immunosuppression of CD8+ T cells.
  • Fig. S4. Tregs cause minor alterations to the mobility of CD8+ T cells in the tumor.
  • Fig. S5. Suppression of CD8+ T cells by Tregs is contact- or proximity-dependent.
  • Fig. S6. Expression of TGF-β and CD51 (αV integrin) in immune cell subsets from the tumors and spleens of B16 tumor–bearing mice.
  • Fig. S7. Effect of DT on the expression of PD-1 and granzyme B on the surface of endogenous CD8+ T cells.
  • Legends for movies S1 and S2

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 789 KB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). CFP-Pmel T cells are found in regions highly infiltrated by Tregs.
  • Movie S2 (.mov format). CFP-Pmel T cells are found within proximity to or make contact with Tregs.

Citation: S. Budhu, D. A. Schaer, Y. Li, R. Toledo-Crow, K. Panageas, X. Yang, H. Zhong, A. N. Houghton, S. C. Silverstein, T. Merghoub, J. D. Wolchok, Blockade of surface-bound TGF-β on regulatory T cells abrogates suppression of effector T cell function in the tumor microenvironment. Sci. Signal. 10, eaak9702 (2017).

© 2017 American Association for the Advancement of Science