Supplementary Materials

Supplementary Materials for:

Protein kinase N3 promotes bone resorption by osteoclasts in response to Wnt5a-Ror2 signaling

Shunsuke Uehara, Nobuyuki Udagawa, Hideyuki Mukai, Akihiro Ishihara, Kazuhiro Maeda, Teruhito Yamashita, Kohei Murakami, Michiru Nishita, Takashi Nakamura, Shigeaki Kato, Yasuhiro Minami, Naoyuki Takahashi, Yasuhiro Kobayashi*

*Corresponding author. Email: ykoba{at}po.mdu.ac.jp

This PDF file includes:

  • Fig. S1. Rankl-induced formation of osteoclasts from Wnt5a−/− liver macrophages on dentin slices.
  • Fig. S2. Micro-CT analysis of femurs and lumbar vertebrae in Ror2ΔOcl/ΔOcl mice and bone-resorbing activity of osteoclasts formed from Ror2ΔOcl/ΔOclmice.
  • Fig. S3. Effects of suppression of Daam2 on osteoclasts.
  • Fig. S4. Effects of shRNA-mediated knockdown of Pkns and mDia2 on osteoclasts.
  • Fig. S5. Micro-CT analysis of femurs and lumbar vertebrae of Pkn3−/− mice.
  • Fig. S6. Osteoclast and osteoblast differentiation in cultures prepared from Pkn3−/− mice.
  • Fig. S7. The expression of c-Src, phosphorylation of Pkn3, and schematic of Pkn3.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.08 MB


Citation: S. Uehara, N. Udagawa, H. Mukai, A. Ishihara, K. Maeda, T. Yamashita, K. Murakami, M. Nishita, T. Nakamura, S. Kato, Y. Minami, N. Takahashi, Y. Kobayashi, Protein kinase N3 promotes bone resorption by osteoclasts in response to Wnt5a-Ror2 signaling. Sci. Signal.10, eaan0023 (2017).

© 2017 American Association for the Advancement of Science