Supplementary Materials

Supplementary Materials for:

Regulation of autophagy, NF-κB signaling, and cell viability by miR-124 in KRAS mutant mesenchymal-like NSCLC cells

Anita K. Mehta, Kevin Hua, William Whipple, Minh-Thuy Nguyen, Ching-Ti Liu, Johannes Haybaeck, Joanne Weidhaas, Jeff Settleman, Anurag Singh*

*Corresponding author. Email: asingh3{at}bu.edu

This PDF file includes:

  • Fig. S1. Regulation of mesenchymal-epithelial transition by KE-associated miRNAs.
  • Fig. S2. Effects of primary MIR-124 lentiviral expression on cellular growth and viability.
  • Fig. S3. Live-cell imaging of autophagic flux in H460 KM cells.
  • Fig. S4. BECN1 expression is suppressed by miR-124.
  • Fig. S5. Repression of p62 and downstream signaling by miR-124.
  • Fig. S6. Control of inflammatory cytokine expression by miR-124.
  • Legends for tables S1 and S2
  • Table S3. Primer and oligo sequences.
  • Legends for movies S1 to S4

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.22 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). TLDA raw data.
  • Table S2 (Microsoft Excel format). TargetScan miR-124 predicted targets.
  • Movie S1 (.avi format). Live-cell imaging of mCherry-GFP-LC3 in control H460 cells.
  • Movie S2 (.avi format). Live-cell imaging of mCherry-GFP-LC3 in miR-124–transfected H460 cells.
  • Movie S3 (.avi format). Live-cell imaging of mCherry-GFP-LC3 in chloroquine-treated H460 cells.
  • Movie S4 (.avi format). Live-cell imaging of mCherry-GFP-LC3 in miR-124 + chloroquine–treated H460 cells.

[Download Tables S1 and S2]


Citation: A. K. Mehta, K. Hua, W. Whipple, M.-T. Nguyen, C.-T. Liu, J. Haybaeck, J. Weidhaas, J. Settleman, A. Singh, Regulation of autophagy, NF-κB signaling, and cell viability by miR-124 in KRAS mutant mesenchymal-like NSCLC cells. Sci. Signal. 10, eaam6291 (2017).

© 2017 American Association for the Advancement of Science