Supplementary Materials

Supplementary Materials for:

Microtubule structures underlying the sarcoplasmic reticulum support peripheral coupling sites to regulate smooth muscle contractility

Harry A. T. Pritchard, Albert L. Gonzales, Paulo W. Pires, Bernard T. Drumm, Eun A. Ko, Kenton M. Sanders, Grant W. Hennig, Scott Earley*

*Corresponding author. Email: searley{at}med.unr.edu

This PDF file includes:

  • Fig. S1. Method used to estimate SR-PM separation distance.
  • Fig. S2. Z-stack analysis of interactions between the SR and microtubule arches.
  • Fig. S3. Microtubules underlie the peripheral SR.
  • Fig. S4. Overlap of RyR2 channel clusters with tubulin.
  • Fig. S5. Nocodazole treatment has no effect on total SR Ca2+ store load or Ca2+ spark frequency.
  • Fig. S6. BKα and RyR2 nearest neighbor analysis.
  • Fig. S7. Actin disruption does not alter STOC frequency.
  • Fig. S8. Inhibition of BK channels increases myogenic tone.
  • Fig. S9. Effects of nocodazole on passive luminal diameter.
  • Legends for movies S1 to S3

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Technical Details

Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Microtubule depolymerization increases the separation distance between the SR and the plasma membrane.
  • Movie S2 (.mov format). Microtubule structure in freshly isolated smooth muscle cells.
  • Movie S3 (.mov format). SR structure in freshly isolated smooth muscle cells.

Citation: H. A. T. Pritchard, A. L. Gonzales, P. W. Pires, B. T. Drumm, E. A. Ko, K. M. Sanders, G. W. Hennig, S. Earley, Microtubule structures underlying the sarcoplasmic reticulum support peripheral coupling sites to regulate smooth muscle contractility. Sci. Signal. 10, eaan2694 (2017).

© 2017 American Association for the Advancement of Science