Supplementary Materials

Supplementary Materials for:

TALK-1 channels control β cell endoplasmic reticulum Ca2+ homeostasis

Nicholas C. Vierra, Prasanna K. Dadi, Sarah C. Milian, Matthew T. Dickerson, Kelli L. Jordan, Patrick Gilon, David A. Jacobson*

*Corresponding author. Email: david.a.jacobson{at}vanderbilt.edu

This PDF file includes:

  • Fig. S1. TALK-1 exhibits ER localization.
  • Fig. S2. Islet cell number and proliferation are not modulated by TALK-1 activity.
  • Fig. S3. Intracellular Ca2+ stores are increased in TALK-1 KO islet cells.
  • Fig. S4. Tetracycline-inducible expression of TALK-1 and TASK-1.
  • Fig. S5. TASK-3 and TASK-1 K2P channel activity alter Ca2+ ER concentrations.
  • Fig. S6. Ca2+ER leak is accelerated by TALK-1 channels.
  • Fig. S7. Hypothetical model depicting potential molecular mechanisms of TALK-1 channel modulation of β cell Ca2+ER handling and Ca2+C oscillations.
  • Table S1. Human islet donor characteristics.
  • Table S2. Human pancreas donor characteristics.
  • Table S3. Primers used for real-time qPCR.

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Citation: N. C. Vierra, P. K. Dadi, S. C. Milian, M. T. Dickerson, K. L. Jordan, P. Gilon, D. A. Jacobson, TALK-1 channels control β cell endoplasmic reticulum Ca2+ homeostasis. Sci. Signal. 10, eaan2883 (2017).

© 2017 American Association for the Advancement of Science