Supplementary Materials

Supplementary Materials for:

Tim-3 signaling in peripheral NK cells promotes maternal-fetal immune tolerance and alleviates pregnancy loss

Yanhong Li, Jiayuan Zhang, Di Zhang, Xiaowu Hong, Yu Tao, Songcun Wang, Yuanyuan Xu, Hailan Piao, Weijie Yin, Min Yu, Yin Zhang, Qiang Fu, Dajin Li, Xing Chang,* Meirong Du*

*Corresponding author. Email: changxing{at}sibs.ac.cn (X.C.); mrdu{at}fudan.edu.cn (M.D.)

This PDF file includes:

  • Fig. S1. Detection of Tim-3 on different cell types in the peripheral blood of patients in early pregnancy.
  • Fig. S2. GO analysis of differentially expressed genes.
  • Fig. S3. Estrogen and β-hCG have no effect on Tim-3 abundance on pNK cells.
  • Fig. S4. The amounts of TGF-β1 and perforin in Tim-3+ and Tim-3 NK cells are unchanged by the BET inhibitor JQ1.
  • Fig. S5. AP mice show decreased Tim-3 and Gal-9 abundance and have dysfunctional NK cells.
  • Fig. S6. The percentage of NK cells in the blood is reduced by RMT3-23.
  • Fig. S7. The function of dNK cells is impaired after Tim-3 blockade.
  • Fig. S8. Tregs are generated in mice that received Tim-3+ NK cells.
  • Fig. S9. Model of how Tim-3 on pNK cells may participate in establishing an immune tolerant phenotype during early pregnancy.

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Citation: Y. Li, J. Zhang, D. Zhang, X. Hong, Y. Tao, S. Wang, Y. Xu, H. Piao, W. Yin, M. Yu, Y. Zhang, Q. Fu, D. Li, X. Chang, M. Du, Tim-3 signaling in peripheral NK cells promotes maternal-fetal immune tolerance and alleviates pregnancy loss. Sci. Signal. 10, eaah4323 (2017).

© 2017 American Association for the Advancement of Science