Supplementary Materials

Supplementary Materials for:

Genome-wide functional analysis reveals central signaling regulators of lymphatic endothelial cell migration and remodeling

Steven P. Williams, Adam F. Odell, Tara Karnezis, Rae H. Farnsworth, Cathryn M. Gould, Jason Li, Sophie Paquet-Fifield, Nicole C. Harris, Anne Walter, Julia L. Gregory, Sara F. Lamont, Ruofei Liu, Elena A. Takano, Cameron J. Nowell, Neil I. Bower, Daniel Resnick, Gordon K. Smyth, Leigh Coultas, Benjamin M. Hogan, Stephen B. Fox, Scott N. Mueller, Kaylene J. Simpson, Marc G. Achen, Steven A. Stacker*

*Corresponding author. Email: steven.stacker{at}petermac.org

This PDF file includes:

  • Fig. S1. Optimization of LEC scratch wound migration assay.
  • Fig. S2. High-throughput scratch wound migration assay.
  • Fig. S3. Genome-wide siRNA screen assay timing.
  • Fig. S4. GO classification of validated regulators of HDLEC migration.
  • Fig. S5. HDLEC morphology cluster phenotypes and corresponding migration scores.
  • Fig. S6. BEC migration assay.
  • Fig. S7. Comparison of selected HMBEC and HDLEC morphology phenotypes.
  • Fig. S8. Validation of a role for LGALS1 in LEC migration and remodeling.
  • Fig. S9. Validation of a role for MICAL2 in LEC migration and remodeling.
  • Fig. S10. Effect of Gal-1 on angiogenesis and blood vessel remodeling.
  • Fig. S11. VEGFR signaling and lineage marker abundance in HDLECs.
  • Fig. S12. Immunofluorescence analysis of LEC and BEC marker proteins in HDLECs.
  • Fig. S13. Immunofluorescence analysis of LEC and common endothelial marker proteins in LGALS1-depleted HDLECs.
  • Legends for data files S1 to S8

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Technical Details

Format: Adobe Acrobat PDF

Size: 3.27 MB

Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). MIARE siRNA screen descriptors.
  • Data file S2 (Microsoft Excel format). Aggregated siRNA screen and LN microarray results.
  • Data file S3 (Microsoft Excel format). Primary screen pathway enrichment analysis.
  • Data file S4 (Microsoft Excel format). Primary screen GO enrichment analysis.
  • Data file S5 (Microsoft Excel format). Secondary screen GO enrichment analysis.
  • Data file S6 (Microsoft Excel format). Secondary screen pathway enrichment analysis.
  • Data file S7 (Microsoft Excel format). Overlapping genes detected in LECs in siRNA screen and LN microarray.
  • Data file S8 (Microsoft Excel format). Overlapping genes detected in LECs in siRNA screen and dermal CHS.

[Download Data files S1 to S8]


Citation: S. P. Williams, A. F. Odell, T. Karnezis, R. H. Farnsworth, C. M. Gould, J. Li, S. Paquet-Fifield, N. C. Harris, A. Walter, J. L. Gregory, S. F. Lamont, R. Liu, E. A. Takano, C. J. Nowell, N. I. Bower, D. Resnick, G. K. Smyth, L. Coultas, B. M. Hogan, S. B. Fox, S. N. Mueller, K. J. Simpson, M. G. Achen, S. A. Stacker, Genome-wide functional analysis reveals central signaling regulators of lymphatic endothelial cell migration and remodeling. Sci. Signal. 10, eaal2987 (2017).

© 2017 American Association for the Advancement of Science