Supplementary Materials

Supplementary Materials for:

Inhibition of the oncogenic fusion protein EWS-FLI1 causes G2-M cell cycle arrest and enhanced vincristine sensitivity in Ewing's sarcoma

Stefan K. Zöllner, Saravana P. Selvanathan, Garrett T. Graham, Ryan M. T. Commins, Sung Hyeok Hong, Eric Moseley, Sydney Parks, Jessica N. Haladyna, Hayriye V. Erkizan, Uta Dirksen, Michael D. Hogarty, Aykut Üren, Jeffrey A. Toretsky*

*Corresponding author. Email: jat42{at}georgetown.edu

This PDF file includes:

  • Fig. S1. YK-4-279 synergizes with vinca alkaloids in ES cells and reduces tumor growth in ES-xenografted mice.
  • Fig. S2. YK-4-279 induces accumulation of presumably mitotic-arrested ES cells while preventing complete chromosomal alignment at metaphase plate.
  • Fig. S3. UBE2C is overexpressed in ES cells and patients, and its depletion sensitizes ES cells to VCR cytotoxicity.
  • Fig. S4. Scoring system for phenotypic changes in spindle architecture, chromosome alignment, and centrosome formation to assess perturbation after single and combinatorial treatments.
  • Fig. S5. RNA-seq data sets of TC32 cells with knockdown of EWS-FLI1 and after treatment with YK-4-279 are consistent with expected ES signatures.
  • Fig. S6. Both knockdown of EWS-FLI1 and treatment with YK-4-279 favor isoform expression of proapoptotic MCL1 and BCL2 transcripts.
  • Fig. S7. YK-4-279 treatment changes the BCLX alternative splicing pattern within the three exons comprising transcript variants.
  • Fig. S8. ES cell lines are resistant to the Bcl-2 inhibitor ABT-737.
  • Table S1. Synergy testing of different drugs combined with YK-4-279 in TC71 cells.
  • Table S2. YK-4-279 displays synergy with vinca alkaloids across different ES cell lines.
  • Table S3. YK-4-279 cuts down on VCR concentration for equivalent cytotoxicity.
  • Table S4. Legend of TCONS.
  • Table S5. List of primers used for qRT-PCR.
  • References (6466)

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Citation: S. K. Zöllner, S. P. Selvanathan, G. T. Graham, R. M. T. Commins, S. H. Hong, E. Moseley, S. Parks, J. N. Haladyna, H. V. Erkizan, U. Dirksen, M. D. Hogarty, A. Üren, J. A. Toretsky, Inhibition of the oncogenic fusion protein EWS-FLI1 causes G2-M cell cycle arrest and enhanced vincristine sensitivity in Ewing's sarcoma. Sci. Signal. 10, eaam8429 (2017).

© 2017 American Association for the Advancement of Science