Supplementary Materials

Supplementary Materials for:

Temperature-activated ion channels in neural crest cells confer maternal fever–associated birth defects

Mary R. Hutson, Anna L. Keyte, Miriam Hernández-Morales, Eric Gibbs, Zachary A. Kupchinsky, Ioannis Argyridis, Kyle N. Erwin, Kelly Pegram, Margaret Kneifel, Paul B. Rosenberg, Pavle Matak, Luke Xie, Jörg Grandl, Erica E. Davis, Nicholas Katsanis, Chunlei Liu,* Eric J. Benner*

*Corresponding author. Email: eric.benner{at}duke.edu (E.J.B); chunlei.liu{at}berkeley.edu (C.L.)

This PDF file includes:

  • Fig. S1. Hyperthermia causes severe craniofacial defects in chicks.
  • Fig. S2. Hyperthermia causes aortic arch patterning defects in chicks.
  • Fig. S3. TRPV channel mRNA expression in neural crest cells.
  • Fig. S4. TRPV1 transcripts are detected in cranial and cardiac neural crest cells by in situ hybridization.
  • Fig. S5. TRPV4 transcripts are detected in cranial and cardiac neural crest cells by in situ hybridization.
  • Fig. S6. Activation of cloned chick TRPV4 and chick TRPV1 with channel-specific ligands.
  • Fig. S7. Neural crest cell–related congenital defects in chicks upon TRPV4 inhibition.
  • Fig. S8. TRPV1 inhibition partially rescues hyperthermia-induced conotruncal defects.
  • Fig. S9. Pharmacological activation of TRPV4 induces fever-related heart defects.
  • Fig. S10. TRPV4 activation disrupts palatogenesis in zebrafish larvae.
  • Fig. S11. TRPV1 maps.
  • Fig. S12. TRPV4 maps.
  • Fig. S13. Expression of FeRIC-modified TRPV1 does not alter cellular Fe2+ homeostasis.
  • Fig. S14. RF coil and SAR estimation.
  • Fig. S15. Membrane integrity and cell viability are unchanged in TRPV1FeRIC-expressing cells after RF stimulation.
  • Fig. S16. Remote activation of FeRIC is ferritin-dependent.
  • Fig. S17. A temperature-insensitive TRPV1 mutant does not respond to RF.
  • Fig. S18. Histological analysis of HH36 hearts confirms conotruncal defects after transient activation of TRPV1FeRIC by RF.
  • Fig. S19. Fever-associated craniofacial defects after remote activation of TRPV1 in neural crest cells.
  • Fig. S20. RF does not cause bulk tissue temperature changes in ovo.
  • Table S1. Structural and functional comparison of GSK101 and GSK1153218 (GSK-dead).
  • Legend for movie S1
  • References (7379)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). MRI reconstruction of a TRPV1FeRIC-induced DORV heart defect in chick.

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Citation: M. R. Hutson, A. L. Keyte, M. Hernández-Morales, E. Gibbs, Z. A. Kupchinsky, I. Argyridis, K. N. Erwin, K. Pegram, M. Kneifel, P. B. Rosenberg, P. Matak, L. Xie, J. Grandl, E. E. Davis, N. Katsanis, C. Liu, E. J. Benner, Temperature-activated ion channels in neural crest cells confer maternal fever–associated birth defects. Sci. Signal. 10, eaal4055 (2017).

© 2017 American Association for the Advancement of Science