Supplementary Materials

Supplementary Materials for:

The matricellular protein TSP1 promotes human and mouse endothelial cell senescence through CD47 and Nox1

Daniel N. Meijles, Sanghamitra Sahoo, Imad Al Ghouleh, Jefferson H. Amaral, Raquel Bienes-Martinez, Heather E. Knupp, Shireen Attaran, John C. Sembrat, Seyed M. Nouraie, Mauricio M. Rojas, Enrico M. Novelli, Mark T. Gladwin, Jeffrey S. Isenberg, Eugenia Cifuentes-Pagano, Patrick J. Pagano*

*Corresponding author. Email: pagano{at}pitt.edu

This PDF file includes:

  • Fig. S1. TSP1 is increased in aging human lung vasculature, and TSP1 exposure reduces endothelial cell proliferation.
  • Fig. S2. Age-dependent changes in senescence marker abundance in mice.
  • Fig. S3. TSP1 does not induce endothelial cell apoptosis.
  • Fig. S4. The TSP1-CD47 axis selectively regulates Nox1.
  • Fig. S5. Nox1 influences cell cycle progression and senescence.
  • Fig. S6. Age-dependent changes in Nox isoform abundance in human and mice lung samples.
  • Table S1. Characteristics of pulmonary disease–free human samples.
  • Table S2. Cause of death and existence of nonpulmonary diseases of the human samples.

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Citation: D. N. Meijles, S. Sahoo, I. Al Ghouleh, J. H. Amaral, R. Bienes-Martinez, H. E. Knupp, S. Attaran, J. C. Sembrat, S. M. Nouraie, M. M. Rojas, E. M. Novelli, M. T. Gladwin, J. S. Isenberg, E. Cifuentes-Pagano, P. J. Pagano, The matricellular protein TSP1 promotes human and mouse endothelial cell senescence through CD47 and Nox1. Sci. Signal. 10, eaaj1784 (2017).

© 2017 American Association for the Advancement of Science