Supplementary Materials

Supplementary Materials for:

Olfactory experience primes the heat shock transcription factor HSF-1 to enhance the expression of molecular chaperones in C. elegans

Felicia K. Ooi and Veena Prahlad*

*Corresponding author. Email: veena-prahlad{at}uiowa.edu

This PDF file includes:

  • Fig. S1. Design and specificity of olfactory pre-exposure and choice assay.
  • Fig. S2. The compound 2AA made by PA14 specifically modulates olfactory avoidance behavior and protects against PA14-induced death.
  • Fig. S3. Serotonin is required for learning-mediated HSF-1 activation.
  • Fig. S4. Characterization of C. elegans HSF-1.
  • Fig. S5. Characterization of C. elegans HSF-1 after exposure to water odor and 2AA odor.
  • Fig. S6. The formation of HSF-1 nuclear bodies does not require RNA pol II.
  • Fig. S7. HSF-1 is required for olfactory learning.
  • Table S1. Survival of animals on PA14 is dependent on HSF-1.
  • Table S2. Statistical analyses.
  • Table S3. 2AA does not appear to be toxic to C. elegans.
  • Table S4. Pre-exposure to the odor of PA14 protects animals from subsequent exposure to PA14.
  • Table S5. Primers used for qRT-PCR analysis.
  • Table S6. Primers used for ChIP-PCR and ChIP-qPCR analysis.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.40 MB


Citation: F. K. Ooi, V. Prahlad, Olfactory experience primes the heat shock transcription factor HSF-1 to enhance the expression of molecular chaperones in C. elegans. Sci. Signal. 10, eaan4893 (2017).

© 2017 American Association for the Advancement of Science