Supplementary Materials

Supplementary Materials for:

TCR-stimulated changes in cell surface CD46 expression generate type 1 regulatory T cells

Siobhan Ni Choileain, Joanne Hay, Joelle Thomas, Anna Williams, Matthieu M. Vermeren, Cecile Benezech, Mario Gomez-Salazar, Owen R. Hugues, Sonja Vermeren, Sarah E. M. Howie, Ian Dransfield, Anne L. Astier*

*Corresponding author. Email: anne.astier{at}inserm.fr

This PDF file includes:

  • Fig. S1. Changes in the molecular mass of CD46 occur mainly in memory CD4+ T cells.
  • Fig. S2. Modulation of sialic acids upon T cell activation.
  • Fig. S3. CD3 stimulation synergizes with CD46 stimulation to induce CD46 shedding.
  • Fig. S4. Chemical inhibition of O-glycosylation decreases the reduction in CD46 abundance upon stimulation and enhances IFN-γ production.
  • Fig. S5. CD46-BC1-GFP and CD46-ΔSTP-GFP are expressed at the cell surface and recognized by the anti-CD46 antibody.
  • Fig. S6. Expression of the ΔSTP mutant CD46 protein reduces the shedding of CD46.
  • Fig. S7. CD4+ T cells expressing either CD46-BC1 or CD46-ΔSTP are equivalently activated by CD3/CD28 stimulation.
  • Fig. S8. The STP domain of CD46 is important for its recruitment to the immune synapse.
  • Fig. S9. BC1-CD46 is recruited to the immune synapse.
  • Fig. S10. The STP domain of CD46 is required for its recruitment to the immune synapse.
  • Fig. S11. Inhibition of the proteasome by MG132 promotes the loss of cell surface CD46.
  • Fig. S12. CD4+ T cells expressing the ΔSTP mutant exhibit enhanced NF-κB activation.
  • Fig. S13. CD4+ T cells from MS patients respond normally to stimulation.
  • Fig. S14. Normalized Cyt1 and Cyt2 abundance in CD4+ T cells from IFN-β–treated and untreated MS patients.

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Citation: S. Ni Choileain, J. Hay, J. Thomas, A. Williams, M. M. Vermeren, C. Benezech, M. Gomez-Salazar, O. R. Hugues, S. Vermeren, S. E. M. Howie, I. Dransfield, A. L. Astier, TCRstimulated changes in cell surface CD46 expression generate type 1 regulatory T cells. Sci. Signal. 10, eaah6163 (2017).

© 2017 American Association for the Advancement of Science