Supplementary Materials

Supplementary Materials for:

TGF-β receptor I/II trafficking and signaling at primary cilia are inhibited by ceramide to attenuate cell migration and tumor metastasis

Salih Gencer, Natalia Oleinik, Jisun Kim, Shanmugam Panneer Selvam, Ryan De Palma, Mohammed Dany, Rose Nganga, Raquela J. Thomas, Can E. Senkal, Philip H. Howe, Besim Ogretmen*

*Corresponding author. Email: ogretmen{at}musc.edu

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  • Fig. S1. CerS4 is down-regulated in most advanced metastatic tumor tissues.
  • Fig. S2. CerS4 knockdown results in increased cell migration and invasion.
  • Fig. S3. CerS4−/− mice exhibit irreversible alopecia and decreased C18- and/or C20-ceramide synthesis.
  • Fig. S4. CerS4 induction increases C18- and C20-ceramide synthesis.
  • Fig. S5. Analysis of Smad7 expression and the subcellular localization of TβRI/II after CerS4 knockdown.
  • Fig. S6. CerS4 knockdown induces Shh abundance without affecting p-Smad3 abundance.
  • Fig. S7. CerS4 knockdown enables cell migration through cross-talk between TβRI/II and Shh/Smo signaling.
  • Fig. S8. Inhibition of primary cilia formation prevents CerS4 knockdown-induced cell migration and metastasis to the liver and lung.

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Citation: S. Gencer, N. Oleinik, J. Kim, S. Panneer Selvam, R. De Palma, M. Dany, R. Nganga, R. J. Thomas, C. E. Senkal, P. H. Howe, B. Ogretmen, TGF-β receptor I/II trafficking and signaling at primary cilia are inhibited by ceramide to attenuate cell migration and tumor metastasis. Sci. Signal. 10, eaam7464 (2017).

© 2017 American Association for the Advancement of Science