Supplementary Materials

Supplementary Materials for:

MARK3-mediated phosphorylation of ARHGEF2 couples microtubules to the actin cytoskeleton to establish cell polarity

María-José Sandí, Christopher B. Marshall, Marc Balan, Étienne Coyaud, Ming Zhou, Daniel M. Monson, Noboru Ishiyama, Arun A. Chandrakumar, José La Rose, Amber L. Couzens, Anne-Claude Gingras, Brian Raught, Wei Xu, Mitsuhiko Ikura, Deborah K. Morrison, Robert Rottapel*

*Corresponding author. Email: rottapel{at}uhnresearch.ca

This PDF file includes:

  • Fig. S1. ARHGEF2 interaction network.
  • Fig. S2. Full alignment of ARHGEF2 orthologs in vertebrates.
  • Fig. S3. Quantitative analysis of the effect of MARK3 phosphorylation of ARHGEF2 on its interactions with DYNLT1 and 14-3-3.
  • Fig. S4. Structural characterization of the DYNLT1-ARHGEF2 interaction.
  • Fig. S5. MARK3, but not PP6, regulates the phosphorylation of ARHGEF2 Ser151.
  • Fig. S6. Generation and validation of inducible cells.
  • Fig. S7. MARK3 affects the biological activity of ARHGEF2.
  • Fig. S8. Phosphorylation of ARHGEF2 Ser151 is required for normal cell polarity.
  • Table S1. Summary of ARHGEF2 interactors reported in this study.
  • Table S2. GO enrichment analysis of the ARHGEF2 network.
  • Table S3. Functional annotation of ARHGEF2 interactors.
  • Table S4. Analysis of MARK3–C-TAK1 complexes.
  • Table S5. Data collection and refinement statistics for the DYNLT1:ARHGEF2 chimera (PDB: 5WI4).

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 4.70 MB

Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Raw data for the ARHGEF2 interactors reported in this study.

Citation: M.-J. Sandí, C. B. Marshall, M. Balan, É. Coyaud, M. Zhou, D. M. Monson, N. Ishiyama, A. A. Chandrakumar, J. La Rose, A. L. Couzens, A.-C. Gingras, B. Raught, W. Xu, M. Ikura, D. K. Morrison, R. Rottapel, MARK3-mediated phosphorylation of ARHGEF2 couples microtubules to the actin cytoskeleton to establish cell polarity. Sci. Signal. 10, eaan3286 (2017).

© 2017 American Association for the Advancement of Science