Supplementary Materials

Supplementary Materials for:

Neuronal activity drives FMRP- and HSPG-dependent matrix metalloproteinase function required for rapid synaptogenesis

Mary L. Dear, Jarrod Shilts, Kendal Broadie*

*Corresponding author. Email: kendal.broadie{at}vanderbilt.edu

This PDF file includes:

  • Fig. S1. Temperature controls for dTRPA1 activity-induced synaptic bouton formation.
  • Fig. S2. Mmp2 is not required for activity-dependent synaptic bouton formation.
  • Fig. S3. Mmp1 is rapidly and specifically increased after dTRPA1 neuronal stimulation.
  • Fig. S4. Synaptic Mmp2 is rapidly reduced after acute neuronal stimulation.
  • Fig. S5. Synaptic Dlp is rapidly increased after acute neuronal stimulation.
  • Fig. S6. Synaptic Mmp1 and Dlp imaging controls at the NMJ terminal.
  • Fig. S7. Synaptic Dlp changes with bidirectional dlp genetic manipulations.
  • Fig. S8. Synaptic Mmp2 changes with bidirectional dlp genetic manipulations.
  • Fig. S9. Activity-dependent synaptic Dlp increase occurs in the absence of Mmp1.
  • Fig. S10. Synaptic Dlp in FXS disease model is restored by single-copy dlp coremoval.

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Citation: M. L. Dear, J. Shilts, K. Broadie, Neuronal activity drives FMRP- and HSPG-dependent matrix metalloproteinase function required for rapid synaptogenesis. Sci. Signal. 10, eaan3181 (2017).

© 2017 American Association for the Advancement of Science