Supplementary Materials

Supplementary Materials for:

Generation of specific inhibitors of SUMO-1– and SUMO-2/3–mediated protein-protein interactions using Affimer (Adhiron) technology

David J. Hughes,* Christian Tiede, Natalie Penswick, Anna Ah-San Tang, Chi H. Trinh, Upasana Mandal, Katarzyna Z. Zajac, Thembaninskosi Gaule, Gareth Howell, Thomas A. Edwards, Jianxin Duan, Eric Feyfant, Michael J. McPherson, Darren C. Tomlinson,* Adrian Whitehouse*

*Corresponding author. Email: djh25{at}st-andrews.ac.uk (D.J.H.); d.c.tomlinson{at}leeds.ac.uk (D.C.T.); a.whitehouse{at}leeds.ac.uk (A.W.)

This PDF file includes:

  • Fig. S1. Structures of proteins used in this study.
  • Fig. S2. Identification of isoform-specific S-Affs.
  • Fig. S3. Cell viability after expression of S-Affs.
  • Fig. S4. S-Affs interact with SUMOylated cellular proteins.
  • Table S1. Crystallographic data collection, processing, and refinement statistics for the SUMO-1:S1S2D5, SUMO-2:S1S2D5, and SUMO-2:S2B3 complexes.
  • Table S2. Key hydrogen bonds predicted from MD simulations of the SUMO-1:S1S2D5, SUMO-2:S1S2D5, SUMO-1:S2B3, SUMO-1-Alt:S2B3, and SUMO-2:S2B3 complexes.
  • References (60)

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Citation: D. J. Hughes, C. Tiede, N. Penswick, A. Ah-San Tang, C. H. Trinh, U. Mandal, K. Z. Zajac, T. Gaule, G. Howell, T. A. Edwards, J. Duan, E. Feyfant, M. J. McPherson, D. C. Tomlinson, A. Whitehouse, Generation of specific inhibitors of SUMO-1– and SUMO-2/3–mediated protein-protein interactions using Affimer (Adhiron) technology. Sci. Signal. 10, eaaj2005 (2017).

© 2017 American Association for the Advancement of Science